Calreticulin expression in neoplastic versus normal dog mammary glands: a cDNA subtraction-based study
| Autor: | Masaru Okuda, Toshiharu Hayashi, Nobuo Sasaki, Yuzo Kurio, Takumi Okawa, Masahiro Morimoto, Takuya Mizuno, Takayuki Nakagawa |
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| Rok vydání: | 2010 |
| Předmět: |
Mammary gland
Blotting Western Molecular Sequence Data Cathepsin A Mammary Neoplasms Animal Biology Adenocarcinoma Real-Time Polymerase Chain Reaction Dogs Cell Line Tumor medicine Biomarkers Tumor Animals alpha-Macroglobulins Dog Diseases RNA Messenger Peptide sequence Gene Library General Veterinary Base Sequence Hyperplasia medicine.disease Molecular biology Lactotransferrin Lactoferrin medicine.anatomical_structure CDNA Subtraction biology.protein Immunohistochemistry Female Calreticulin |
| Zdroj: | Research in veterinary science. 92(1) |
| ISSN: | 1532-2661 |
| Popis: | This is the first report describing the expression of canine calreticulin (cCRT) in canine mammary gland tumour (MGT). Using cDNA subtraction method, it is found that mRNAs of CRT, cathepsin A, ovostatin, and lactotransferrin were differentially expressed in mammary adenocarcinoma as compared to hyperplasia, both of which were obtained from the dog. Furthermore, the mRNA expression levels of CRT and cathepsin A were significantly higher in canine MGT samples than in nontumour samples. In contrast, immunohistochemical studies have indicated that the expression of cCRT protein found to be detected in most of mammary gland tissues and was not correlated to the types of canine MGTs. Furthermore, cCRT was molecularly cloned, and the amino acid sequence of cCRT was found to be very similar to those of other species. Further studies are required to elucidate additional roles of cCRT in canine MGT. |
| Databáze: | OpenAIRE |
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