Rescue of human monoclonal antibody production from an EBV-transformed B cell line by fusion to a human-mouse hybridoma
| Autor: | Dianne M. Fishwild, Steven K. H. Foung, J. Larrick, EdgarG. Engleman, Susan Perkins, G E Lizak, F. C. Grumet, Andrew Raubitschek |
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| Rok vydání: | 1984 |
| Předmět: |
Herpesvirus 4
Human medicine.drug_class Immunology Monoclonal antibody Lymphocyte Activation ABO Blood-Group System Cell Line Cell Fusion Epitopes Mice Antigen medicine Immunology and Allergy Animals Humans Secretion Antibody-Producing Cells B cell B-Lymphocytes Cell fusion Hybridomas biology Chemistry Antibodies Monoclonal Cell Transformation Viral Virology Molecular biology medicine.anatomical_structure Cell culture Monoclonal biology.protein Antibody |
| Zdroj: | Journal of immunological methods. 70(1) |
| ISSN: | 0022-1759 |
| Popis: | A human-mouse cell line that is hypoxanthine-aminopterin-thymidine sensitive and ouabain resistant was derived from a fusion between human B lymphocytes and a mouse myeloma line. This new mutant, when fused to a relatively unstable EBV-transformed B cell secreting a human monoclonal anti-A (red blood cell antigen) antibody, resulted in stable hybridomas capable of long term production of the specific human monoclonal antibody. Furthermore, some of the hybrid clones secreted antibody in far greater titer than the original EBV cell line. We conclude that fusion to this human-mouse line is an efficient approach to the production of human monoclonal alloantibodies and an effective method of 'rescuing' secretion of desired antibody from EBV cell lines. |
| Databáze: | OpenAIRE |
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