Restoration of Corneal Transparency by Mesenchymal Stem Cells

Autor: Afsaneh Amouzegar, Alexandra Rezazadeh, Srikant K Sahu, Masahiro Omoto, Anuradha Sahu, Sharad K. Mittal, Kishore Reddy Katikireddy, Dhvanit I. Shah, Sunil K. Chauhan
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
medicine.medical_specialty
genetic structures
Interleukin-1beta
Gene Expression
Ocular trauma
Biology
Mesenchymal Stem Cell Transplantation
Biochemistry
Cell Line
Cornea
Mice
03 medical and health sciences
0302 clinical medicine
Report
Ophthalmology
Genetics
medicine
Animals
Humans
Regeneration
RNA
Small Interfering
lcsh:QH301-705.5
Wound Healing
lcsh:R5-920
Hepatocyte Growth Factor
Regeneration (biology)
Mesenchymal stem cell
Mesenchymal Stem Cells
Cell Biology
Fibroblasts
eye diseases
Corneal transparency
030104 developmental biology
medicine.anatomical_structure
lcsh:Biology (General)
Cell culture
Models
Animal
030221 ophthalmology & optometry
Hepatocyte growth factor
sense organs
Wound healing
lcsh:Medicine (General)
Corneal Injuries
Developmental Biology
medicine.drug
Zdroj: Stem Cell Reports, Vol 7, Iss 4, Pp 583-590 (2016)
Stem Cell Reports
ISSN: 2213-6711
Popis: Summary Transparency of the cornea is indispensable for optimal vision. Ocular trauma is a leading cause of corneal opacity, leading to 25 million cases of blindness annually. Recently, mesenchymal stem cells (MSCs) have gained prominence due to their inflammation-suppressing and tissue repair functions. Here, we investigate the potential of MSCs to restore corneal transparency following ocular injury. Using an in vivo mouse model of ocular injury, we report that MSCs have the capacity to restore corneal transparency by secreting high levels of hepatocyte growth factor (HGF). Interestingly, our data also show that HGF alone can restore corneal transparency, an observation that has translational implications for the development of HGF-based therapy.
Graphical Abstract
Highlights • Inflammation drives MSCs to secrete elevated levels of hepatocyte growth factor (HGF) • MSC-derived HGF restores corneal transparency by inhibiting myofibroblast generation • HGF alone can restore corneal transparency
Ocular injury is a major cause of loss of corneal transparency, leading to blindness. Chauhan and colleagues’ data demonstrate that, following ocular injury, corneal transparency can be restored by MSCs, a process that is completely dependent upon HGF secretion by MSCs. Most importantly, they show that HGF alone can restore corneal transparency, a finding that offers a cell-free HGF-based therapeutic approach.
Databáze: OpenAIRE
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