Suspected‐target and non‐targeted screenings of phosphodiesterase 5 inhibitors in herbal remedies using liquid chromatography–quadrupole time‐of‐flight–mass spectrometry
| Autor: | Ahmad Yusri Mohd Yusop, Linda Xiao, Shanlin Fu |
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| Rok vydání: | 2020 |
| Předmět: |
Spectrometry
Mass Electrospray Ionization Analyte Non targeted Relative standard deviation Pharmaceutical Science Mass spectrometry 01 natural sciences Dosage form Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Limit of Detection Tandem Mass Spectrometry Environmental Chemistry Medicine 030216 legal & forensic medicine Quadrupole time of flight Chromatography High Pressure Liquid Spectroscopy Detection limit Chromatography Reverse-Phase Chromatography business.industry 010401 analytical chemistry Reproducibility of Results Phosphodiesterase 5 Inhibitors 0104 chemical sciences cGMP-specific phosphodiesterase type 5 Plant Preparations Drug Contamination business |
| Zdroj: | Drug Testing and Analysis. 13:965-976 |
| ISSN: | 1942-7611 1942-7603 |
| Popis: | The lucrative market of herbal remedies spurs rampant adulteration, particularly with pharmaceutical drugs and their unapproved analogues. A comprehensive screening strategy is, therefore, warranted to detect these adulterants and, accordingly, to safeguard public health. This study uses the data-dependent acquisition of liquid chromatography-quadrupole time-of-flight-mass spectrometry (LC-QTOF-MS) to screen phosphodiesterase 5 (PDE5) inhibitors in herbal remedies using suspected-target and non-targeted strategies. For the suspected-target screening, we used a library comprising 95 PDE5 inhibitors. For the non-targeted screening, we adopted top-down and bottom-up approaches to flag novel PDE5 inhibitor analogues based on common fragmentation patterns. LC-QTOF-MS was optimised and validated for capsule and tablet dosage forms using 23 target analytes, selected to represent different groups of PDE5 inhibitors. The method exhibited excellent specificity and linearity with limit of detection and limit of quantification of |
| Databáze: | OpenAIRE |
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