Urokinase Receptor Mediates Osteogenic Differentiation of Mesenchymal Stem Cells and Vascular Calcification via the Complement C5a Receptor
| Autor: | Kerstin Jurk, Hermann Haller, Sergey Tkachuk, Inna Dumler, Gregor Theilmeier, Margret Patecki, Jan Larmann, Parnian Kalbasi Anaraki |
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| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
MAP Kinase Signaling System Cellular differentiation Complement receptor Biology Receptors Urokinase Plasminogen Activator Mice Original Research Reports Downregulation and upregulation Osteogenesis Internal medicine medicine Animals Humans Gene silencing Vascular Calcification Receptor Receptor Anaphylatoxin C5a Cells Cultured Mice Knockout Osteoblasts Mesenchymal stem cell NF-kappa B Cell Differentiation Mesenchymal Stem Cells Cell Biology Hematology Cell biology Mice Inbred C57BL Urokinase receptor Endocrinology LDL receptor Developmental Biology |
| Zdroj: | Stem Cells and Development. 23:352-362 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2013.0318 |
| Popis: | Vascular calcification is a severe consequence of several pathological processes with a lack of effective therapy. Recent studies suggest that circulating and resident mesenchymal stem cells (MSC) contribute to the osteogenic program of vascular calcification. Molecular mechanisms underlying MSC osteogenic potential and differentiation remain, however, sparsely explored. We investigated a role for the complement receptor C5aR in these processes. We found that expression of C5aR was upregulated upon differentiation of human MSC to osteoblasts. C5aR inhibition by silencing and specific antagonist impaired osteogenic differentiation. We demonstrate that C5aR expression upon MSC differentiation was regulated by the multifunctional urokinase receptor (uPAR). uPAR targeting by siRNA resulted in complete abrogation of C5aR expression and consequently in the inhibition of MSC-osteoblast differentiation. We elucidated the NFκB pathway as the mechanism utilized by the uPAR-C5aR axis. MSC treatment with the NFκB inhibitor completely blocked the differentiation process. Nuclear translocation of the p65 RelA component of the NFκB complex was induced under osteogenic conditions and impaired by the inhibition of uPAR or C5aR. Dual-luciferase reporter assays demonstrated enhanced NFκB signaling upon MSC differentiation, whereas uPAR and C5aR downregulation lead to inhibition of the NFκB activity. We show involvement of the Erk1/2 kinase in this cascade. In vivo studies in a uPAR/LDLR double knockout mouse model of diet-induced atherosclerosis revealed impaired C5aR expression and calcification in aortic sinus plaques in uPAR(-/-)/LDLR(-/-) versus uPAR(+/+)/LDLR(-/-) control animals. These results suggest that uPAR-C5aR axis via the underlying NFκB transcriptional program controls osteogenic differentiation with functional impact on vascular calcification in vivo. |
| Databáze: | OpenAIRE |
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