Reduction of transforming growth factor-β type II receptor is caused by the enhanced ubiquitin-dependent degradation in human renal cell carcinoma
| Autor: | Takayuki Hattori, Tatsuo Yamamoto, Seiichiro Ozono, Tatsuya Takayama, Chiharu Uchida, Hirotaka Fukasawa, Yoshihide Fujigaki, Sayuri Suzuki, Masatoshi Kitagawa, Kyoko Kitagawa, Soichi Mugiya, Taro Misaki, Naro Ohashi, Toshiaki Oda, Akira Hishida, Hidetoshi Hayashi |
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| Rok vydání: | 2010 |
| Předmět: |
Cancer Research
medicine.medical_specialty Ubiquitin-Protein Ligases Immunoblotting Down-Regulation Protein Serine-Threonine Kinases urologic and male genital diseases Ubiquitin Downregulation and upregulation Transforming Growth Factor beta Renal cell carcinoma Internal medicine medicine Humans RNA Messenger RNA Neoplasm Receptor Carcinoma Renal Cell Smad4 Protein biology Receptor Transforming Growth Factor-beta Type II Ubiquitination medicine.disease Immunohistochemistry Kidney Neoplasms female genital diseases and pregnancy complications Ubiquitin ligase Gene Expression Regulation Neoplastic Endocrinology Oncology biology.protein Cancer research Signal transduction Receptors Transforming Growth Factor beta Signal Transduction Transforming growth factor |
| Zdroj: | International Journal of Cancer. 127:1517-1525 |
| ISSN: | 0020-7136 |
| Popis: | Although dysregulation of transforming growth factor-beta (TGF-beta) signaling is implicated in renal carcinogenesis, its precise mechanism is unknown in renal cell carcinoma (RCC). In our study, we investigated Smad-mediated TGF-beta signaling pathway and its regulatory mechanisms in surgical samples from patients with RCC. We found that immunoreactivity for nuclear phosphorylated Smad2 was significantly decreased in RCC compared to normal renal tissues, thereby TGF-beta signaling was suggested to be attenuated in RCC tissues. In accordance with the result, transcriptional downregulation of Smad4 and post-transcriptional downregulation of TGF-beta type II receptor (TbetaR-II) were frequently found in RCC tissues compared to normal renal tissues. Next, to clarify the reason why the protein level of TbetaR-II was decreased in RCC, we investigated the activities of degradation and ubiquitination of TbetaR-II. We found that both proteasome-mediated degradation and ubiquitination of TbetaR-II were markedly enhanced in RCC tissues. Moreover, we found that the level of Smad-ubiquitination regulatory factor 2 (Smurf2), the E3 ligase for TbetaR-II, was increased in RCC tissues of the patients with higher clinical stages compared to the normal tissues and was inversely correlated with the level of TbetaR-II. Our results suggest that the low TbetaR-II protein level is due to augmented ubiquitin-dependent degradation via Smurf2 and might be involved in the attenuation of TGF-beta signaling pathway in RCC. |
| Databáze: | OpenAIRE |
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