Histological Classification and Immunohistochemical Study of Feline Colorectal Epithelial Tumors
| Autor: | Ko Nakashima, Kazuyuki Uchida, James K. Chambers, Hiroyuki Nakayama, Mizuho Uneyama |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Adenoma
Pathology medicine.medical_specialty 040301 veterinary sciences Colorectal cancer medicine.medical_treatment Adenocarcinoma Cat Diseases medicine.disease_cause Malignancy 0403 veterinary science 03 medical and health sciences Cytokeratin Biomarkers Tumor Carcinoma medicine Animals 030304 developmental biology 0303 health sciences General Veterinary business.industry 04 agricultural and veterinary sciences medicine.disease Adenocarcinoma Mucinous Immunohistochemistry Cats Female Lymphadenectomy KRAS Colorectal Neoplasms business |
| Zdroj: | Veterinary Pathology. 58:305-314 |
| ISSN: | 1544-2217 0300-9858 |
| DOI: | 10.1177/0300985820974279 |
| Popis: | Among 113 feline gastrointestinal epithelial tumors diagnosed between 2006 and 2019, 78 (69%) were detected in the colorectum. Fifty colorectal tumors were selected for further pathological evaluations, of which 9 (18%) were histopathologically diagnosed as adenomas and 41 (82%) as carcinoma. The carcinomas included 33 tubular adenocarcinomas (TAC), 5 tubulovillous adenocarcinomas (TVAC), 2 mucinous adenocarcinomas, and 1 undifferentiated carcinoma. Histopathologically, TAC frequently showed vascular invasion (17/33 cases, 52%). In TAC cases, serosal infiltration (13/15 cases, 87%) and lymph node metastasis (8/9 cases, 89%) were common in bowel resection and lymphadenectomy samples, respectively. Immunohistochemically, the tumor cells of most cases were positive for cytokeratin (CK) 20 (50/50 cases, 100%) and CDX2 (48/50 cases, 96%). Focal immunopositivity for CD10 (11/50 cases, 22%) and CK7 (15/50 cases, 30%) was observed irrespective of the histological subtype. Only a few cases showed diffuse nuclear accumulation of β-catenin (2/50 cases, 4%) and p53 (5/50 cases, 10%). A lack of tubule formation, female sex, and low CDX2 labeling were statistically associated with carcinoma compared to adenoma (ρ = 0.615, P < .001; ρ = 0.279, P = .050; and ρ = −0.265, P = .063, respectively). Other features, including mucin profiles, Ki67 labeling index, and accumulation of β-catenin and p53, were not associated with malignancy. A sequence analysis revealed KRAS mutations in 3/7 TAC cases. These results suggest that KRAS mutations—rather than excessive Wnt/β-catenin signaling and the inactivation of TP53—contribute to the tumorigenesis of feline colorectal carcinoma. |
| Databáze: | OpenAIRE |
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