Counterimmunoelectrophoresis test for immunoglobulin M antibodies to group B coxsackievirus
| Autor: | D B Nelson, D J D'Alessio, T E Minor, P. B. Helstrom |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Microbiology (medical)
Counterimmunoelectrophoresis Coxsackievirus Infections Heterologous Biology Coxsackievirus Antibodies Viral biology.organism_classification Precipitin Virology Enterovirus B Human Diagnosis Differential Immunoglobulin M Antigen Antibody Specificity Neutralization Tests biology.protein Antibody Neutralizing antibody Immunoelectrophoresis Mercaptoethanol Research Article |
| Zdroj: | Journal of Clinical Microbiology. 9:503-506 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.9.4.503-506.1979 |
| Popis: | A counterimmunoelectrophoresis test was developed for immunoglobulin M (IgM) antibodies to group B coxsackievirus (CB) types 1 through 5. The IgM precipitin line could be identified and differentiated from the IgG line by treating sera with 2-mercaptoethanol. Antigen purity was demonstrated by single precipitin lines occurring only to the homologous antigen when tested with type-specific hyperimmune rabbit sera. Serum pairs from 19 of 22 patients with documented CB type 1,3,4, and 5 infections were positive for IgM antibody to the infecting serotype, whereas 2 of 7 pairs from CB type 2 patients were positive. Heterologous IgM antibodies were present in sera from 14 fo 29 CB patients. Of the 14 patients with heterologous IgM antibodies, 12 also had greater than or equal to 4-fold rises in whole serum neutralizing antibody to heterologous serotypes. Only three control sera from 72 patients with coxsackievirus group A, echovirus, or other viral infections had IgM antibody to CB serotypes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|