Effects of moderate dietary supplementations with n — 3 fatty acids on macrophage and lymphocyte phospholipids and macrophage eicosanoid synthesis in the rat
| Autor: | Christophe Brouard, Marc Pascaud |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Male
Leukotrienes Linseed Oil food.ingredient Linoleic acid Biophysics 6-Ketoprostaglandin F1 alpha Arachidonic Acids Biology Biochemistry Dinoprostone Membrane Lipids chemistry.chemical_compound Fish Oils Endocrinology food Dietary Fats Unsaturated Linseed oil Animals Lymphocytes Food science Peritoneal Cavity Phospholipids Unsaturated fatty acid chemistry.chemical_classification Arachidonic Acid Macrophages Rats Inbred Strains Fish oil Eicosapentaenoic acid Rats Thromboxane B2 Eicosapentaenoic Acid chemistry Eicosanoid Eicosanoids lipids (amino acids peptides and proteins) Arachidonic acid Spleen Polyunsaturated fatty acid |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. 1047:19-28 |
| ISSN: | 0005-2760 |
| DOI: | 10.1016/0005-2760(90)90255-v |
| Popis: | The effects of a moderate dietary intake of n-3 polyunsaturated fatty acids were determined on the eicosanoid synthesis by rat peritoneal macrophages: the availability of arachidonic acid (20:4 (n-6)) and eicosapentaenoic acid (20:5 (n-3] in the membrane phospholipids of peritoneal macrophages and splenic lymphocytes and the synthesis of the main eicosanoids were evaluated in parallel. The n-6/n-3 ratio was decreased from 13.5 in the control diet rich in 18:2(n-6) to about 2 by an addition of either linseed oil providing 18:3(n-3) (linseed oil diet) or fish oil providing 20:5(n-3) and 22:6(n-3) (fish oil diet). The dietary linoleic acid content was at least 3.5% of energy in each diet. In peritoneal macrophage and splenic lymphocyte membrane phospholipids, arachidonic acid content was significantly decreased with the linseed oil and fish oil diets. 20:5(n-3) content was significantly raised up in the linseed oil group (3-fold in macrophage phospholipids) and more strikely in the fish oil group (7-fold). In response to a stimulation by the calcium ionophore A23187, peritoneal macrophages released 6-ketoPGF1 alpha, TXB2, PGE2 and LTB4 and/or 5, but no or few peptidoleukotrienes. The linseed oil did not affect significantly the synthesis of these eicosanoids, except for LTB5, which was increased 2-fold. In contrast, the fish oil diet led to significant decreases in the productions of 6-ketoPGF1 alpha and PGE2 (-50%) and LTB4 (-40%) and to a 10-fold increase in the release of LTB5. TXB2 was also decreased (-35%), but not significantly. These results and the parallel evolutions of the two ratios 20:4(n-6)/20:5(n-3) and LTB4/LTB5 showed that eicosanoid synthesis is greatly dependent on the relative availability of the two substrates in competition. |
| Databáze: | OpenAIRE |
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