Endothelial cell migration on surfaces modified with immobilized adhesive peptides
| Autor: | Rena Bizios, Stylianos Kouvroukoglou, Kyriacos Zygourakis, Kay C Dee, Larry V. McIntire |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Cell
Biophysics Motility Bioengineering Video microscopy Peptide Biomaterials chemistry.chemical_compound Cell Movement medicine Cell Adhesion Animals Amino Acid Sequence Cell adhesion Cells Cultured chemistry.chemical_classification Chemistry Dansyl chloride Adhesion Endothelial stem cell medicine.anatomical_structure Biochemistry Mechanics of Materials Ceramics and Composites Cattle Endothelium Vascular Peptides |
| Zdroj: | Biomaterials. 21(17) |
| ISSN: | 0142-9612 |
| Popis: | Endothelial cell (EC) migration has been studied on aminophase surfaces with covalently bound RGDS and YIGSRG cell adhesion peptides. The fluorescent marker dansyl chloride was used to quantify the spatial distribution of the peptides on the modified surfaces. Peptides appeared to be distributed in uniformly dispersed large clusters separated by areas of lower peptide concentrations. We employed digital time-lapse video microscopy and image analysis to monitor EC migration on the modified surfaces and to reconstruct the cell trajectories. The persistent random walk model was then applied to analyze the cell displacement data and compute the mean root square speed, the persistence time, and the random motility coefficient of EC. We also calculated the time-averaged speed of cell locomotion. No differences in the speed of cell locomotion on the various substrates were noted. Immobilization of the cell adhesion peptides (RGDS and YIGSRG), however, significantly increased the persistence of cell movement and, thus, the random motility coefficient. These results suggest that immobilization of cell adhesion peptides on the surface of implantable biomaterials may lead to enhanced endothelization rates. |
| Databáze: | OpenAIRE |
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