E-cadherin and plakoglobin recruit plakophilin3 to the cell border to initiate desmosome assembly
| Autor: | Samrat T. Kundu, Nileema Khapare, Lalit Sehgal, Mansi S. Karkhanis, Sorab N. Dalal, Prajakta Gosavi |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Cell
Fluorescent Antibody Technique Plakoglobin chemistry.chemical_element Biology Calcium Cell Line Cellular and Molecular Neuroscience Desmosome Cell Adhesion medicine Humans Molecular Biology Pharmacology Gene knockdown Microscopy Confocal Cadherin Desmosomes Cell Biology Adhesion Cadherins HCT116 Cells Cell biology HEK293 Cells medicine.anatomical_structure chemistry Gene Knockdown Techniques Desmosome assembly Molecular Medicine gamma Catenin Plakophilins |
| Zdroj: | Cellular and Molecular Life Sciences. 68:1439-1454 |
| ISSN: | 1420-9071 1420-682X |
| DOI: | 10.1007/s00018-010-0531-3 |
| Popis: | A decrease in the levels of the desmosomal plaque protein, plakophilin3 (PKP3), leads to a decrease in desmosome size and cell-cell adhesion. To test the hypothesis that PKP3 is required for desmosome formation, the recruitment of desmosomal components to the cell surface was studied in the PKP3 knockdown clones. The PKP3 knockdown clones showed decreased cell border staining for multiple desmosomal proteins, when compared to vector controls, and did not form desmosomes in a calcium switch assay. Further analysis demonstrated that PKP3, plakoglobin (PG) and E-cadherin are present at the cell border at low concentrations of calcium. Loss of either PG or E-cadherin led to a decrease in the levels of PKP3 and other desmosomal proteins at the cell border. The results reported here are consistent with the model that PG and E-cadherin recruit PKP3 to the cell border to initiate desmosome formation. |
| Databáze: | OpenAIRE |
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