Cross-reactive proteins among eight bovine mycoplasmas detected by monoclonal antibodies
| Autor: | Barik A. Salih, Ricardo F. Rosenbusch |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
medicine.drug_class
Dispar Immunoblotting Immunology Cattle Diseases Mycoplasmataceae Cross Reactions medicine.disease_cause Monoclonal antibody Microbiology Mycoplasma Bacterial Proteins Antigen Antigenic variation medicine Animals Immunology and Allergy Mycoplasma Infections Antigens Bacterial General Veterinary biology Antibodies Monoclonal Membrane Proteins General Medicine biology.organism_classification Molecular biology Molecular Weight Infectious Diseases Mollicutes biology.protein Cattle Electrophoresis Polyacrylamide Gel Rabbits Antibody |
| Zdroj: | Comparative Immunology, Microbiology and Infectious Diseases. 24:103-111 |
| ISSN: | 0147-9571 |
| Popis: | Whole cell proteins of eight bovine mycoplasmas (M. bovoculi, M. bovis, M. dispar, M. bovirhinis, M. arginini, M. verecundum, M. canadense, M. alkalescens) were separated by SDS-PAGE and transferred to nitrocellulose paper. Rabbit anti-M. bovoculi serum was found to react with immunoblots of all mycoplasma species tested. These cross-reactive proteins were in the range of 35,000-100,000 molecular weight. Monoclonal antibody MA25.5 developed against a M. bovoculi 94 kDa surface protein cross-reacted with a band of 62 kDa from M. dispar and three bands of 89, 85 and 74 kDa from M. arginini only while MA18.13 that recognized a band of 57 kDa from M. bovoculi did not react with the other species. The role of MA25.5 monoclonal antibody in inhibiting the growth of M. bovoculi, M. dispar and M. arginini was tested using the metabolic-inhibition (MI) test. Monoclonal antibody MA25.5 inhibited the growth of M. bovoculi and also inhibited M. dispar growth but at lower MI titers, while it showed no effect on the growth of M. arginini. |
| Databáze: | OpenAIRE |
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