Cytokinins are pervasive among common in vitro culture media: An analysis of their forms, concentrations and potential sources
| Autor: | Anna Kisiala, Megan M. Aoki, Tamzida Rahman, Erin N. Morrison, R. J. Neil Emery |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cytokinins Microorganism Bioengineering Biology 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound Tissue culture Plant Growth Regulators 010608 biotechnology Animals Yeast extract Organism Growth medium General Medicine In vitro Culture Media 030104 developmental biology Biochemistry chemistry Tryptone Cell culture Biotechnology |
| Zdroj: | Journal of Biotechnology. 334:43-46 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2021.05.005 |
| Popis: | The number of organisms that are known to produce cytokinins (CKs) continues to increase. In fact, species from all life kingdoms have now been shown to either produce CKs or at least have the genetic components to make it possible. In vitro growth of microorganisms, plant/animal cells, and tissue cultures often requires nutrient-rich media composed of ingredients with organic origins including: yeast extract, peptone, tryptone, or various plant or animal extracts. These compounds, derived from microbial, plant and animal materials, can be the source of significant levels of exogenous CKs in the culture medium. As CK investigative work continues to expand rapidly, it is of critical importance to draw attention to this complexity; the presence of CKs in growth medium affects CK metabolism of the cultured organism and interferes with the readings of analytical instrumentation used to profile CKs in tested microorganisms or cell cultures. |
| Databáze: | OpenAIRE |
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