Identification of a gene at 16q24.3 that restores cellular senescence in immortal mammary tumor cells
| Autor: | Arbansjit K. Sandhu, Gursurinder P. Kaur, Raghbir S. Athwal, Jon K. deRiel, Deepthi E Reddy |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Senescence
Cancer Research Breast Neoplasms Biology Adenocarcinoma medicine.disease_cause Chromosome 16 Gene mapping Genetics medicine Tumor Cells Cultured Animals Humans Molecular Biology Gene Cellular Senescence Mutation Chromosome Mammary Neoplasms Experimental Physical Chromosome Mapping Cell biology Rats Genetic marker Carcinogenesis Cell Division Chromosomes Human Pair 16 |
| Zdroj: | Oncogene. 18(36) |
| ISSN: | 0950-9232 |
| Popis: | We have mapped a cellular senescence gene, SEN16, within a genetic distance of 3 – 7 cM, at 16q24.3. Microcell mediated transfer of a normal human chromosome 16, 16q22-qter or 16q23-qter restored cellular senescence in four immortal cell lines, derived from human and rat mammary tumors. The resumption of indefinite cell proliferation, concordant with the segregation of the donor chromosome, confirmed the presence of a senescence gene at 16q23-qter. While microcell hybrids were maintained in selection medium to retain the donor chromosome, sporadic immortal revertant clones arose among senescent cells. Reversion to immortal growth could occur due to inactivation of the senescence gene either by a mutation or a deletion. The analysis for chromosome 16 specific DNA markers, in revertant clones of senescent microcell hybrids, revealed a consensus deletion, spanning a genetic interval of approximately 3 – 7 cM at 16q24.3. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|