MMP-10/stromelysin-2 promotes invasion of head and neck cancer
| Autor: | Hirotaka Tani, Samadarani B. S. M. Siriwardena, Shinji Iizuka, Takashi Takata, Mohammad Reza Kiekhaee, Maki Yoshida, Yasusei Kudo, Elsayed Mohamed Deraz, Mariko Obayashi, Lorenzo Lo Muzio, Takaaki Tsunematsu, Yoshimitsu Abiko, Akira Kikuchi, Guangying Qi, Ikuko Ogawa, Giuseppina Campisi |
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| Přispěvatelé: | Deraz, EM, Kudo, Y, Yoshida, M, Obayashi, M, Tsunematsu, T, Tani, H, Siriwardena, SB, Keikhaee, MR, Qi, G, Iizuka, S, Ogawa, I, Campisi, G, Lo Muzio, L, Abiko, Y, Kikuchi, A, Takata, T |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Tumor Physiology
Clone (cell biology) p38 Mitogen-Activated Protein Kinases Metastasis Molecular Cell Biology Basic Cancer Research Neoplasm Metastasis Regulation of gene expression Gene knockdown Multidisciplinary Head and Neck cancer Transfection Head and Neck Tumors Extracellular Matrix Up-Regulation Gene Expression Regulation Neoplastic Oncology Head and Neck Neoplasms Gene Knockdown Techniques Carcinoma Squamous Cell Medicine Research Article Science Oral Medicine Biology Periostin Head and Neck Squamous Cell Carcinoma Matrix Metalloproteinase 10 stomatognathic system Settore MED/28 - Malattie Odontostomatologiche Cell Line Tumor medicine Cancer Detection and Diagnosis otorhinolaryngologic diseases Humans Neoplasm Invasiveness Extracellular Matrix Adhesions Protein Kinase Inhibitors neoplasms Microarray analysis techniques Cancers and Neoplasms medicine.disease Molecular biology Head and neck squamous-cell carcinoma Antigens Differentiation Wnt Proteins stomatognathic diseases Cancer research Cell Adhesion Molecules |
| Zdroj: | PLoS ONE, Vol 6, Iss 10, p e25438 (2011) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | BackgroundPeriostin, IFN-induced transmembrane protein 1 (IFITM1) and Wingless-type MMTV integration site family, member 5B (Wnt-5b) were previously identified as the invasion promoted genes of head and neck squamous cell carcinoma (HNSCC) by comparing the gene expression profiles between parent and a highly invasive clone. We have previously reported that Periostin and IFITM1 promoted the invasion of HNSCC cells. Here we demonstrated that Wnt-5b overexpression promoted the invasion of HNSCC cells. Moreover, stromelysin-2 (matrix metalloproteinase-10; MMP-10) was identified as a common up-regulated gene among Periostin, IFITM1 and Wnt-5b overexpressing HNSCC cells by using microarray data sets. In this study, we investigated the roles of MMP-10 in the invasion of HNSCC.Methods and findingsWe examined the expression of MMP-10 in HNSCC cases by immunohistochemistry. High expression of MMP-10 was frequently observed and was significantly correlated with the invasiveness and metastasis in HNSCC cases. Next, we examined the roles of MMP-10 in the invasion of HNSCC cells in vitro. Ectopic overexpression of MMP-10 promoted the invasion of HNSCC cells, and knockdown of MMP-10 suppressed the invasion of HNSCC cells. Moreover, MMP-10 knockdown suppressed Periostin and Wnt-5b-promoted invasion. Interestingly, MMP-10 overexpression induced the decreased p38 activity and MMP-10 knockdown induced the increased p38 activity. In addition, treatment with a p38 inhibitor SB203580 in HNSCC cells inhibited the invasion.ConclusionsThese results suggest that MMP-10 plays an important role in the invasion and metastasis of HNSCC, and that invasion driven by MMP-10 is partially associated with p38 MAPK inhibition. We suggest that MMP-10 can be used as a marker for prediction of metastasis in HNSCC. |
| Databáze: | OpenAIRE |
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