A rapid diffusion immunoassay in a T-sensor
| Autor: | Anson Hatch, Andrew Kamholz, Paul Yager, Kenneth R. Hawkins, Eric A. Schilling, Bernhard H. Weigl, Matthew S. Munson |
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| Rok vydání: | 2001 |
| Předmět: |
Analyte
Diffusion Microfluidics Biomedical Engineering Bioengineering Antigen-Antibody Complex Binding Competitive Applied Microbiology and Biotechnology Antibody Specificity Fluorescence Polarization Immunoassay medicine Whole blood Immunoassay Chromatography medicine.diagnostic_test Chemistry Small molecule Molecular Weight Phenytoin Reagent Molecular Medicine Rheology Quantitative analysis (chemistry) Biotechnology |
| Zdroj: | Nature Biotechnology. 19:461-465 |
| ISSN: | 1546-1696 1087-0156 |
| DOI: | 10.1038/88135 |
| Popis: | We have developed a rapid diffusion immunoassay that allows measurement of small molecules down to subnanomolar concentrations in1 min. This competitive assay is based on measuring the distribution of a labeled probe molecule after it diffuses for a short time from one region into another region containing antigen-specific antibodies. The assay was demonstrated in the T-sensor, a simple microfluidic device that places two fluid streams in contact and allows interdiffusion of their components. The model analyte was phenytoin, a typical small drug molecule. Clinically relevant levels were measured in blood diluted from 10- to 400-fold in buffer containing the labeled antigen. Removal of cells from blood samples was not necessary. This assay compared favorably with fluorescence polarization immunoassay (FPIA) measurements. Numerical simulations agree well with experimental results and provide insight for predicting assay performance and limitations. The assay is homogeneous, requires1 microl of reagents and sample, and is applicable to a wide range of analytes. |
| Databáze: | OpenAIRE |
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