Characterization of the major allergens purified from the venom of the paper wasp Polistes gallicus
| Autor: | Gloriano Moneti, M. Severino, Stefano Turillazzi, Giampaolo Manao, Giuseppe Orsomando, Donald R. Hoffman, Gianni Cappugi, Barbara Pantera, L. Carresi, Luigia Pazzagli, Igino Spadolini |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Hypersensitivity
Immediate Models Molecular Protein Conformation Wasp Venoms Molecular Sequence Data Wasps Biophysics Venom Cross Reactions medicine.disease_cause Biochemistry Vespula Microbiology Allergen Species Specificity Polistes annularis medicine Animals Humans Amino Acid Sequence Anaphylaxis Molecular Biology Phylogeny Paper wasp biology Allergens biology.organism_classification Immunology Polistes Polistes gallicus |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - General Subjects. 1623:72-81 |
| ISSN: | 0304-4165 |
| DOI: | 10.1016/j.bbagen.2003.07.001 |
| Popis: | Allergic reactions to vespid stings are one of the major causes of IgE-mediated anaphylaxis. Vespa and Vespula venoms are closely related; Polistes venom is more distantly related and its allergens are less well studied. There is limited cross-reactivity between Polistes and the other vespid venoms because of differences in the epitopes on the allergen molecules. In this study, the major allergens of Polistes gallicus are isolated and characterized. P. gallicus venom contains four major allergens: phospholipase, antigen 5 (Ag5), hyaluronidase and protease that were characterized by mass spectrometry and specific binding to IgE. The complete amino acid sequence of Ag5 and the sequence of the N-terminal region of phospholipase were also determined. The alignment of Ag5 from P. gallicus (European species) and Polistes annularis (American species) shows an 85% identity that increases to 98% within the same subgenus. This could suggest the presence of specific epitopes on Ag5 molecule being the variations on the superficial loops. The features of the P. gallicus allergens could explain the partial cross-reactivity found between the American and European Polistes venoms, and suggest that the use of European Polistes venoms would improve the diagnostic specificity and the therapy of European patients and of North American patients sensitized by European Polistes. |
| Databáze: | OpenAIRE |
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