Transcription Factor YY1 Interacts with Retroviral Integrases and Facilitates Integration of Moloney Murine Leukemia Virus cDNA into the Host Chromosomes▿
| Autor: | Shinji Iijima, Kazuhito Imamura, Yuya Kinoshita, Ken-ichi Nishijima, Katsuhide Miyake, Yujin Inayoshi, Toshinari Itoh, Ken Kono, Yuuki Okino, Yusuke Morimoto, Mahboob Morshed, Junko Yamamoto-Kishikawa, Akifumi Mizutani |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
DNA
Complementary viruses Virus Integration Immunology Viral transformation Biology Cell Fractionation Microbiology Avian sarcoma virus Virus Viral Proteins Virology Murine leukemia virus Protein Interaction Mapping Humans Immunoprecipitation YY1 Transcription Factor Gammaretrovirus Integrases biology.organism_classification Molecular biology humanities Integrase Genome Replication and Regulation of Viral Gene Expression Avian Sarcoma Viruses Insect Science Gene Knockdown Techniques DNA Viral biology.protein HIV-1 Moloney murine leukemia virus Protein Binding |
| Popis: | Retroviral integrases associate during the early viral life cycle with preintegration complexes that catalyze the integration of reverse-transcribed viral cDNA into the host chromosomes. Several cellular and viral proteins have been reported to be incorporated in the preintegration complex. This study demonstrates that transcription factor Yin Yang 1 binds to Moloney murine leukemia virus, human immunodeficiency virus type 1, and avian sarcoma virus integrases. The results of coimmunoprecipitation and in vitro pulldown assays revealed that Yin Yang 1 interacted with the catalytic core and C-terminal domains of Moloney murine leukemia virus and human immunodeficiency virus type 1 integrases, while the transcriptional repression and DNA-binding domains of the Yin Yang 1 molecule interacted with Moloney murine leukemia virus integrase. Immunoprecipitation of the cytoplasmic fraction of virus-infected cells followed by Southern blotting and chromatin immunoprecipitation demonstrated that Yin Yang 1 associated with Moloney murine leukemia virus cDNA in virus-infected cells. Yin Yang 1 enhanced the in vitro integrase activity of Moloney murine leukemia virus, human immunodeficiency virus type 1, and avian sarcoma virus integrases. Furthermore, knockdown of Yin Yang 1 in host cells by small interfering RNA reduced Moloney murine leukemia virus cDNA integration in vivo , although viral cDNA synthesis was increased, suggesting that Yin Yang 1 facilitates integration events in vivo . Taking these results together, Yin Yang 1 appears to be involved in integration events during the early viral life cycle, possibly as an enhancer of integration. |
| Databáze: | OpenAIRE |
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