Hydrogen peroxide induces heme oxygenase-1 and dentin sialophosphoprotein mRNA in human pulp cells
| Autor: | Sun-Kyung Lee, Hong-In Shin, Suk-Keun Lee, Kyung-San Min, Hyung-Ryong Kim, Suk-Ho Kim, Eun-Cheol Kim, Hwa-Jeong Lee, Hun-Taeg Chung, Hyun-Ock Pae |
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| Rok vydání: | 2008 |
| Předmět: |
Materials science
Cell Survival MAP Kinase Signaling System Cellular differentiation Sialoglycoproteins Blotting Western Odontoblast differentiation stomatognathic system Dentin sialophosphoprotein Tooth Bleaching Humans Viability assay RNA Messenger Protein kinase A General Dentistry Cells Cultured Dental Pulp Extracellular Matrix Proteins Odontoblasts Kinase Reverse Transcriptase Polymerase Chain Reaction Cell Differentiation Hydrogen Peroxide Oxidants Phosphoproteins Molecular biology Heme oxygenase stomatognathic diseases Oxidative Stress Cytoprotection Pulp (tooth) Heme Oxygenase-1 |
| Zdroj: | Journal of endodontics. 34(8) |
| ISSN: | 1878-3554 |
| Popis: | Although the induction of heme oxygenase-1 (HO-1) by hydrogen peroxide (H2O2) has been reported, the HO-1 and odontoblastic differentiation-inducing effects against H2O2 have not been clarified in human pulp cells. In this study, we investigated whether HO-1 is involved in the protective mechanisms against the cytotoxic effects of H2O2 by using a cell viability assay, and we examined the production of dentin sialophosphoprotein (DSPP) and other mineralization markers by using reverse transcriptase-polymerase chain reaction in human pulp cells. H2O2 decreased cell viability but increased HO-1 and DSPP expression in a concentration- and time-dependent manner. Antioxidants and inhibitors of HO-1, phosphatidylinositol-3'-kinase, extracellular signal-regulated kinase, and p38 mitogen-activated protein kinase blocked H2O2-induced cytotoxicity and the expression of HO-1 and DSPP mRNA in pulp cells. These data suggest that the induction of HO-1 by H2O2 in pulp cells plays a protective role against the cytotoxic effects of H2O2 and stimulates DSPP expression, resulting in premature odontoblast differentiation through pathways that involve phosphatidylinositol-3'-kinase, p38, and extracellular signal-regulated kinase. |
| Databáze: | OpenAIRE |
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