Safety assessment of a modified acetolactate synthase protein (GM-HRA) used as a selectable marker in genetically modified soybeans
| Autor: | Jean Schmidt, Gabrielle Carlson, S. Ballou, J.F. Barnett, Bryan Delaney, L.M. Appenzeller, Raymond Layton, Carey A. Mathesius, Gregory S. Ladics, John Zhang, J. Ding, Robert F. Cressman, Carol Carpenter |
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| Rok vydání: | 2009 |
| Předmět: |
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Hot Temperature Food Genetically Modified Genetically modified crops Biology Toxicology Mice Enzyme Stability Toxicity Tests Animals Amino Acid Sequence Peptide sequence Selectable marker chemistry.chemical_classification Mice Inbred ICR Acetolactate synthase Gastric Juice Dose-Response Relationship Drug Intestinal Secretions Computational Biology Agriculture General Medicine Plants Genetically Modified Amino acid Genetically modified organism Acetolactate Synthase Enzyme chemistry Biochemistry Glycine biology.protein Female Soybeans Biotechnology Herbicide Resistance |
| Zdroj: | Regulatory Toxicology and Pharmacology. 55:309-320 |
| ISSN: | 0273-2300 |
| DOI: | 10.1016/j.yrtph.2009.08.003 |
| Popis: | Acetolactate synthase (ALS) enzymes have been isolated from numerous organisms including soybeans (Glycine max; GM-ALS) and catalyze the first common step in biosynthesis of branched chain amino acids. Expression of an ALS protein (GM-HRA) with two amino acid changes relative to native GM-ALS protein in genetically modified soybeans confers tolerance to herbicidal active ingredients and can be used as a selectable transformation marker. The safety assessment of the GM-HRA protein is discussed. Bioinformatics comparison of the amino acid sequence did not identify similarities to known allergenic or toxic proteins. In vitro studies demonstrated rapid degradation in simulated gastric fluid ( |
| Databáze: | OpenAIRE |
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