Transgenic potato plants expressing mammalian 2'-5' oligoadenylate synthetase are protected from potato virus X infection under field conditions
| Autor: | P. Seppanen, Merja Mehto, Mart Saarma, Jarmo Honkanen, Teemu H. Teeri, Merike Kelve, Anja Hassi, Reetta Puska, Anu Aaspöllu, E. Truve |
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| Rok vydání: | 1993 |
| Předmět: |
0106 biological sciences
Transgene Molecular Sequence Data Biomedical Engineering Gene Expression Sequence Homology Bioengineering Genetically modified crops Biology Transfection 01 natural sciences Applied Microbiology and Biotechnology Virus Plant Viruses 03 medical and health sciences Mice Complementary DNA 2' 5'-Oligoadenylate Synthetase Animals Amino Acid Sequence 030304 developmental biology Solanum tuberosum 2. Zero hunger 0303 health sciences 2'-5'-Oligoadenylate fungi food and beverages DNA Potato virus X biology.organism_classification Potexvirus Blotting Northern Plants Genetically Modified Virology Rats Transformation (genetics) Blotting Southern Molecular Medicine 010606 plant biology & botany Biotechnology Plasmids |
| Zdroj: | Bio/technology (Nature Publishing Company). 11(9) |
| ISSN: | 0733-222X |
| Popis: | We cloned and sequenced a rat cDNA encoding the 2'-5' oligoadenylate synthetase, a component of the mammalian interferon-induced antiviral response, and used Agrobacterium-mediated transformation to generate transgenic potato clones expressing this mammalian enzyme. In transgenic plants infected with potato virus X and followed under field conditions, virus concentrations in leaves and in tubers were significantly lower than in nontransgenic controls. Additionally, virus concentration in the leaves of five transgenic clones and in tubers of one clone was also lower than in transgenic potatoes expressing potato virus X coat protein. |
| Databáze: | OpenAIRE |
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