Pharmacokinetics of cytisine after single intravenous and oral administration in rabbits
| Autor: | Nikolay Danchev, Dobrin Svinarov, Lilia V Kassabova, Henri Angel Astroug, Roumiana Simeonova |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Pharmacology
Detection limit rabbits business.industry Health Toxicology and Mutagenesis cytisine Absorption (skin) Toxicology Quantitative determination Pharmacokinetic analysis Cytisine chemistry.chemical_compound HPLC method Pharmacokinetics chemistry Oral administration Medicine Original Article Hplc method business pharmacokinetics |
| Zdroj: | Interdisciplinary Toxicology |
| ISSN: | 1337-9569 1337-6853 |
| Popis: | The aim of this study is to develop a sensitive HPLC method for the quantitative determination of cytisine in serum and to characterize the pharmacokinetic behaviour of cytisine after oral and intravenous administration in rabbits. The pharmacokinetic behaviour of cytisine is studied in male and female New Zealand rabbits after oral and intravenous administration. Cytisine is administered orally (dose of 5 mg/kg b.w.) under fasting condition (12 hours) and intravenously (dose 1 mg/kg b.w.) in the marginal ear vein. Cytisine serum concentrations are measured using a highly selective and sensitive validated HPLC method with UV detection. Linearity of the method is in the range 12–2 400 µg/L; accuracy and precision are both within ± 10%, and the limit of detection is 4 µg/L. Selectivity and stability are also validated. Basic pharmacokinetic parameters of cytisine after single oral and intravenous administration are calculated using TOPFIT software. Pharmacokinetic analysis suggests a rapid but incomplete absorption of cytisine after oral administration. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|