An amperometric cellobiose dehydrogenase-based biosensor can be used for measurement of cellulase activity
| Autor: | Lars H. Eng, Gunnar Johansson, Göran Pettersson, Sten-Eric Lindqvist, Lars Hildén |
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| Rok vydání: | 2001 |
| Předmět: |
Quality Control
Cellobiose dehydrogenase Time Factors Biophysics Cellobiose Cellulase Biosensing Techniques Phanerochaete Biochemistry chemistry.chemical_compound Hydrolysis Glucose oxidase Molecular Biology Chromatography biology Chemistry Reproducibility of Results Cell Biology biology.organism_classification Amperometry Evaluation Studies as Topic biology.protein Carbohydrate Dehydrogenases Biosensor |
| Zdroj: | Analytical biochemistry. 290(2) |
| ISSN: | 0003-2697 |
| Popis: | The hemoflavoenzyme cellobiose dehydrogenase (CDH, EC 1.1.99.18) from Phanerochaete chrysosporium has been used in an amperometric redox polymer-based biosensor. Used in conjugation with a FIA system this biosensor can replace colorimetric assays for measuring cellobiose liberated from cellulose in a series of cellulase-containing samples. The biosensor gave the same result as the Somogyi-Nelson method in a less time-consuming and laborious manner. The two methods showed about the same precision. |
| Databáze: | OpenAIRE |
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