Hyposplenism: Comparison of different methods for determining splenic function
| Autor: | Jan van Marle, Joost B. L. Hoekstra, J. Carel Goslings, Ester M. M. van Leeuwen, Ineke J. M. ten Berge, Peter Speelman, Roelof J. Bennink, A. J. Jolanda Lammers, Bart J. Biemond, Alexander P. N. A. de Porto |
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| Přispěvatelé: | Other departments, Amsterdam institute for Infection and Immunity, Amsterdam Gastroenterology Endocrinology Metabolism, Cancer Center Amsterdam, Nuclear Medicine, Experimental Immunology, Clinical Haematology, Amsterdam Movement Sciences, Surgery, Nephrology, Amsterdam Cardiovascular Sciences, General Internal Medicine |
| Rok vydání: | 2012 |
| Předmět: |
Adult
Male Splenic function Erythrocytes Anemia Phagocytosis Erythrocytes Abnormal Spleen scintigraphy Anemia Sickle Cell Sickle Cell Trait Young Adult Humans Medicine Antigens Young adult Radionuclide Imaging Aged Sodium Pertechnetate Tc 99m Splenic Diseases Pneumococcal sepsis business.industry Vaccination Organ Size Hematology Middle Aged medicine.disease Lymphocyte Subsets Normal functioning Erythrocyte Inclusions Splenic Tissue Antibody Formation Immunology Splenectomy Female business Immunologic Memory Spleen |
| Zdroj: | American journal of hematology, 87(5), 484-489. Wiley-Liss Inc. |
| ISSN: | 0361-8609 |
| DOI: | 10.1002/ajh.23154 |
| Popis: | Asplenic patients are at risk for pneumococcal sepsis. Patients with hyposplenic function, such as associated with sickle cell disease (SCD), are also at risk. However, tests to assess splenic function are either unavailable or lacking standardization. The aim of this study was to compare different methods for determining splenic function. Eighteen patients with SCD (i.e., 10 heterozygous (SC) and 8 homozygous (SS) SCD patients), and eight splenectomized patients were compared to 10 controls. All subjects underwent spleen scintigraphy, after which functional splenic volumes (FSV) were calculated. FSV was compared to immunological function and B cell-subsets, as well as phagocytic function represented by the presence of Howell Jolly bodies (HJB) and percentages of pitted red cells (PIT). Heterozygous SCD (SC) patients had increased splenic volumes, but diminished FSV, homozygous SCD (SS) patients were asplenic. Splenectomized and SS patients had a strongly reduced phagocytic and immunological function. SC patients had reduced anti-polysaccharide responses without an increase in PIT. FSV correlated significantly with phagocytic and immunological function. HJB were indicative of splenic dysfunction, HJB absence was not indicative of normal functioning splenic tissue. Although visualizing HJB is methodologically advantageous to PIT, both are valid biomarkers of splenic dysfunction. The amount of non-switched memory B cells is strongly correlated to FSV. Am. J. Hematol. 87:484489, 2012. (c) 2012 Wiley Periodicals, Inc |
| Databáze: | OpenAIRE |
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