Designing Artificial Fluorescent Proteins: Squaraine‐LmrR Biophosphors for High Performance Deep‐Red Biohybrid Light‐Emitting Diodes

Autor: Sara Ferrara, Sara H. Mejias, Mantas Liutkus, Giacomo Renno, Francesca Stella, Irene Kociolek, Juan Pablo Fuenzalida‐Werner, Claudia Barolo, Pedro B. Coto, Aitziber L. Cortajarena, Rubén D. Costa
Přispěvatelé: European Commission, Fundação para a Ciência e a Tecnologia (Portugal), European Research Council, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Technical University of Munich
Rok vydání: 2022
Předmět:
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
Advanced Functional Materials
ISSN: 1616-3028
1616-301X
DOI: 10.1002/adfm.202111381
Popis: Biophosphors with fluorescent proteins (FPs) are promising candidates to replace rare-earth color down-converting filters for white light-emitting diodes (LEDs). There is, however, a lack of deep-red FPs meeting high photostabilities, photoluminescence quantum yields (ϕ), and throughput expression yields. Herein, a new approach for the design of highly emissive and stable deep-red biophosphors combining an artificial FP (Lactococcal multidrug resistance Regulator (LmrR) as protein host and an archetypal red-emitting squaraine (S) as guest) with a polymer network is demonstrated toward high performing deep-red biohybrid LEDs (Bio-HLEDs). At first, the best protein pocket (aromaticity, polarity, charge, etc.) to stabilize S in water is determined using four LmrR variants (position 96 with tryptophan, histidine, phenylalanine, and alanine). Computational and time-resolved spectroscopic findings suggest that the tryptophan is instrumental toward achieving artificial red-emitting FPs with ϕ > 50% stable over weeks. These features are further enhanced in the polymer coating (ϕ > 65% stable over months) without affecting emission color. Finally, deep-red Bio-HLEDs are fabricated featuring external quantum efficiencies of 7% and stabilities of ≈800 h. This represents threefold enhancement compared to reference devices with S-polymer color filters. Overall, this work highlights a new design for highly emissive deep-red biophosphors, achieving record performance in deep-red protein-LEDs.
The authors acknowledge the European Union's Horizon 2020 research and innovation FET-OPEN under grant agreement ARTIBLED No. 863170. R.D.C. acknowledges the ERC-Co InOutBioLight No. 816856. P.B.C. acknowledges financial support from the Ministry of Science, Innovation and Universities of Spain under the Beatriz Galindo Programme (No. MCIU-19-BEAGAL 18/0224), from MCIN/AEI/10.13039/501100011033 grant No. PGC2018-095953-B-I00 and from the Technical University of Munich (TUM) under the TUM Global Visiting Professor Programme. A.L.C. acknowledges support by the European Research Council ERC-CoG-648071-ProNANO and ERC-PoC-841063-NIMM; and Agencia Estatal de Investigación, Spain (No. PID2019-111649RB-I00). This work was performed under the Maria de Maeztu Units of Excellence Program from the Spanish State Research Agency Grant No. MDM-2017-0720 (CIC biomaGUNE).
Databáze: OpenAIRE
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