Increased Mitochondrial-Encoded Gene Transcription in Immortal DF-1 Cells
| Autor: | Farris James A, Douglas N. Foster, In Jeong Kim, Hyunggee Kim, Linda K. Foster, Seungkwon You |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
animal structures
Transcription Genetic Mitochondrial translation Chick Embryo Biology DNA Mitochondrial Adenosine Triphosphate Transcription (biology) Animals Humans Respiratory function Gene Cell Line Transformed Protein Synthesis Inhibitors Differential display Cell growth Gene Expression Profiling Cell Cycle Cell Biology Fibroblasts Blotting Northern Molecular biology Mitochondria Cell biology Kinetics Chloramphenicol Gene Expression Regulation Mitochondrial biogenesis Cell culture Dactinomycin Cell Division |
| Zdroj: | Experimental Cell Research. 265:339-347 |
| ISSN: | 0014-4827 |
| DOI: | 10.1006/excr.2001.5207 |
| Popis: | We have established, in continuous cell culture, a spontaneously immortalized chicken embryo fibroblast (CEF) cell line (DF-1) as well as several other immortal CEF cell lines. The immortal DF-1 cells divided more rapidly than primary and other immortal CEF cells. To identify the genes involved in rapidly dividing DF-1 cells, we have used differential display RT-PCR. Of the numerous genes analyzed, three mitochondrial-encoded genes (ATPase 8/6, 16S rRNA, and cytochrome b) were shown to express at higher levels in DF-1 cells compared to primary and other immortal CEF cells. The inhibition of mitochondrial translation by treatment with chloramphenicol markedly decreased ATP production and cell proliferation in DF-1 cells, while not affecting growth in either primary or other immortal CEF cells. This result suggests a correlation between rapid cell proliferation and the increased mitochondrial respiratory functions. We also determined that the increased transcription of mitochondrial-encoded genes in DF-1 cells is due to increased de novo transcript synthesis as shown by mitochondrial run-on assays, and not the result of either increased mitochondrial biogenesis or mitochondrial transcript half-lives. Together, the present studies suggest that the transcriptional activation of mitochondrial-encoded genes and the elevated respiratory function should be one of the characteristics of rapidly dividing immortal cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect