Temperature-dependence of open-complex formation at two Escherichia coli promoters with extended -10 sequences
| Autor: | Stephen D. Minchin, Tamara A. Belyaeva, Helen D. Burns, Stephen J. W. Busby |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Transcription Genetic Stereochemistry Complex formation Molecular Sequence Data DNA Footprinting Sequence (biology) Random hexamer Biology medicine.disease_cause Biochemistry chemistry.chemical_compound Potassium Permanganate RNA polymerase Operon medicine Escherichia coli Promoter Regions Genetic Molecular Biology Base Sequence DNA Superhelical Temperature Galactose Promoter Cell Biology DNA-Directed RNA Polymerases Methyltransferases Molecular biology chemistry Nucleic Acid Conformation DNA Research Article |
| Popis: | We have studied the formation of open complexes between purified RNA polymerase from Escherichia coli and DNA fragments carrying the galP1 promoter, a promoter with an extended -10 region. Unusually, these complexes are formed readily at low temperatures. This low-temperature opening is unaffected by deletions of either upstream or downstream promoter sequences. We conclude that low-temperature open-complex formation is due to specific base sequences in and just upstream of the extended -10 region. In contrast, open complexes are not formed at low temperatures with DNA fragments carrying the E. coli cysG promoter, which also has an extended -10 region. This demonstrates that an extended -10 sequence alone is not sufficient for low-temperature opening. Additionally, we report the temperature dependence of a hybrid galP1–cysG promoter, the related galP2 and galP3 promoters and a derivative of galP1 with an improved -10 hexamer sequence. |
| Databáze: | OpenAIRE |
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