Fe3O4-Au core-shell nanoparticles as a multimodal platform for in vivo imaging and focused photothermal therapy
| Autor: | Ana M. Beltrán, Alejandro Domínguez, Manuel Leal, Carlos Caro, John R. Pearson, María L. García-Martín, Yilian Fernández-Afonso, Pedro Quaresma, Jesús M. de la Fuente, Ricardo Franco, Jose María Páez-Muñoz, Eulália Pereira, Francisco Gámez |
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| Přispěvatelé: | UCIBIO - Applied Molecular Biosciences Unit, DQ - Departamento de Química, Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica, Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. FQM102: Estereoquímica y Síntesis Asimétrica, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, [Caro,C, Páez-Muñoz,JM, Domínguez,A, Pearson,JR, García-Martín,ML] BIONAND—Centro Andaluz de Nanomedicina y Biotecnología (Junta de Andalucía-Universidad de Málaga), Málaga, Spain. [Gámez,F] Departamento de Química Física, Universidad de Granada, Granada, Spain. [Quaresma,P: Pereira,E] REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências da Universidade do Porto, Porto, Portugal. [Pernía Leal,M] Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad de Sevilla, Seville, Spain. [Beltrán,AM] Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Escuela Politécnica Superior, Universidad de Sevilla, Sevilla, Spain. [Fernandez-Afonso,Y, De la Fuente,JM] Instituto de Nanociencia y Materiales de Aragón (INMA), CSIC-Universidad de Zaragoza, Zaragoza, Spain. [De la Fuente,JM, García-Martín,ML] Biomedical Research Networking Center in Bioengineering, Biomaterials &Nanomedicine (CIBER-BBN), Madrid, Spain [Franco,R] UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade NOVA de Lisboa, Caparica, Portugal., Financial support was provided by the Spanish Ministry of Economy, Industry and Competitiveness (CTQ2017-86655-R and BIO2017-84246-C2-1-R), Fondo Social de la DGA (grupos DGA) and by the Regional Ministry of Health of Andalusia (OH-0026-2018). This work was supported by the Associate Laboratory for Green Chemistry—LAQV, and by the Applied Molecular Biosciences Unit –UCIBIO, both financed by Portuguese national funds from FCT/MCTES (UIDB/04378/2020 and UIDB/50006/2020)., Ministerio de Economía, Industria y Competitividad (España), Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Diputación General de Aragón, Junta de Andalucía, Fundação para a Ciência e a Tecnologia (Portugal), Ministério da Ciência, Tecnologia e Ensino Superior (Portugal) |
| Rok vydání: | 2021 |
| Předmět: |
Imagen por resonancia magnética
Phenomena and Processes::Metabolic Phenomena::Pharmacokinetics::Tissue Distribution [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Magnetic Resonance Imaging [Medical Subject Headings] Nanoparticle Pharmaceutical Science lcsh:RS1-441 multimodal imaging 02 engineering and technology TomografíacComputarizada por rayos X 01 natural sciences Phenomena and Processes::Biological Phenomena::Ecological and Environmental Phenomena::Environment::Atmosphere::Weather::Temperature::Hot Temperature [Medical Subject Headings] chemistry.chemical_compound Organisms::Eukaryota::Animals [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Multimodal Imaging [Medical Subject Headings] Zebrafish Organisms::Eukaryota::Animals::Chordata::Vertebrates::Fishes::Cypriniformes::Cyprinidae::Zebrafish [Medical Subject Headings] Analytical Diagnostic and Therapeutic Techniques and Equipment::Investigative Techniques::Chemistry Techniques Analytical::Surface Plasmon Resonance [Medical Subject Headings] Technology and Food and Beverages::Technology Industry and Agriculture::Manufactured Materials::Nanostructures::Nanoparticles::Metal Nanoparticles [Medical Subject Headings] Temperature Nanopartículas Imaging-guided therapy Temperatura Diseases::Neoplasms [Medical Subject Headings] contrast agent 021001 nanoscience & nanotechnology Photothermal therapy Imagen multimodal Neoplasias Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia [Medical Subject Headings] imaging-guided therapy Tissues Contrast agent 0210 nano-technology Preclinical imaging Iron oxide nanoparticles MRI CT Terapia fototérmica Biodistribution Biocompatibility Medios de contraste Analytical Diagnostic and Therapeutic Techniques and Equipment::Diagnosis::Diagnostic Techniques and Procedures::Diagnostic Imaging::Tomography::Tomography X-Ray::Tomography X-Ray Computed [Medical Subject Headings] Pez cebra Technology and Food and Beverages::Technology Industry and Agriculture::Manufactured Materials::Biomedical and Dental Materials::Polymers::Plastics::Polyvinyls::Povidone [Medical Subject Headings] 010402 general chemistry Article lcsh:Pharmacy and materia medica Therapeutic index In vivo Multimodal imaging Organisms::Eukaryota::Animals::Chordata::Vertebrates::Mammals::Rodentia::Muridae::Murinae::Mice [Medical Subject Headings] Chemicals and Drugs::Chemical Actions and Uses::Pharmacologic Actions::Diagnostic Uses of Chemicals::Contrast Media [Medical Subject Headings] 0104 chemical sciences Tejidos chemistry Nanoparticles Biomedical engineering |
| Zdroj: | Zaguán: Repositorio Digital de la Universidad de Zaragoza Universidad de Zaragoza Digibug: Repositorio Institucional de la Universidad de Granada Universidad de Granada (UGR) Pharmaceutics Pharmaceutics, Vol 13, Iss 416, p 416 (2021) Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Digibug. Repositorio Institucional de la Universidad de Granada instname Zaguán. Repositorio Digital de la Universidad de Zaragoza idUS. Depósito de Investigación de la Universidad de Sevilla Digital.CSIC. Repositorio Institucional del CSIC Volume 13 Issue 3 idUS: Depósito de Investigación de la Universidad de Sevilla Universidad de Sevilla (US) |
| Popis: | In this study, we report the synthesis of gold-coated iron oxide nanoparticles capped with polyvinylpyrrolidone (Fe@Au NPs). The as-synthesized nanoparticles (NPs) exhibited good stability in aqueous media and excellent features as contrast agents (CA) for both magnetic resonance imaging (MRI) and X-ray computed tomography (CT). Additionally, due to the presence of the local surface plasmon resonances of gold, the NPs showed exploitable “light-to-heat” conversion ability in the near-infrared (NIR) region, a key attribute for effective photothermal therapies (PTT). In vitro experiments revealed biocompatibility as well as excellent efficiency in killing glioblastoma cells via PTT. The in vivo nontoxicity of the NPs was demonstrated using zebrafish embryos as an intermediate step between cells and rodent models. To warrant that an effective therapeutic dose was achieved inside the tumor, both intratumoral and intravenous routes were screened in rodent models by MRI and CT. The pharmacokinetics and biodistribution confirmed the multimodal imaging CA capabilities of the Fe@AuNPs and revealed constraints of the intravenous route for tumor targeting, dictating intratumoral administration for therapeutic applications. Finally, Fe@Au NPs were successfully used for an in vivo proof of concept of imaging-guided focused PTT against glioblastoma multiforme in a mouse model. Spanish Ministry of Economy, Industry and Competitiveness (CTQ2017-86655-R and BIO2017-84246-C2-1-R) Fondo Social de la DGA (grupos DGA) and by the Regional Ministry of Health of Andalusia (OH-0026-2018). Associate Laboratory for Green Chemistry—LAQV Applied Molecular Biosciences Unit –UCIBIO Portuguese national funds from FCT/MCTES (UIDB/04378/2020 and UIDB/50006/2020) |
| Databáze: | OpenAIRE |
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