Experimental acute biliary pancreatitis induced by retrograde infusion of bile acids into the mouse pancreatic duct
| Autor: | Johanna M. Laukkarinen, Gijs J.D. Van Acker, George Perides, Michael L. Steer |
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| Rok vydání: | 2010 |
| Předmět: |
Taurocholic Acid
medicine.medical_specialty medicine.drug_class Gastroenterology General Biochemistry Genetics and Molecular Biology Bile Acids and Salts Mice Reference Values Small animal Internal medicine medicine Animals Biliary pancreatitis Infusions Parenteral Pancreatic duct Bile acid Extramural business.industry Pancreatic Ducts medicine.disease Disease Models Animal medicine.anatomical_structure Pancreatitis Reference values Acute pancreatitis business Taurolithocholic Acid |
| Zdroj: | Nature protocols. 5(2) |
| ISSN: | 1750-2799 |
| Popis: | Mechanistic studies of acute pancreatitis require animal models because clinical material is generally not available during the early phases of the disease. Here we describe a protocol to induce biliary pancreatitis by retrogradely infusing bile acids into the pancreatic duct of anesthetized mice. The resulting model replicates events believed to be responsible for the onset of clinical biliary (i.e., gallstone) pancreatitis and creates highly reproducible pancreatitis with a severity that depends on the concentration of infused bile acid. Pancreatitis reaches its maximal level of severity within 24 h of induction, and it resolves over the subsequent week. This protocol enables the investigator to use genetically modified strains of mice, and it requires only relatively simple and easily learned techniques of small animal surgery. With practice and gentle technique, the surgery (from induction of anesthesia to completion of the infusion) can be completed within 25 min per animal. |
| Databáze: | OpenAIRE |
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