Mechanical loading of tissue engineered skeletal muscle prevents dexamethasone induced myotube atrophy
| Autor: | Andrew J. Capel, Kathryn W. Aguilar‐Agon, Neil R.W. Martin, Darren J. Player, Jacob W. Fleming, Mark P. Lewis |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Physiology Muscle Fibers Skeletal Skeletal muscle Biochemistry Dexamethasone Muscle hypertrophy Cell Line 03 medical and health sciences Mice 0302 clinical medicine Atrophy Internal medicine medicine Animals Muscle Skeletal Original Paper business.industry Myogenesis Ubiquitin–proteasome Muscle weakness Cell Biology Hypertrophy medicine.disease Muscle atrophy Muscular Atrophy 030104 developmental biology Endocrinology medicine.anatomical_structure Myotubes C2C12 medicine.symptom business 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Journal of Muscle Research and Cell Motility |
| ISSN: | 1573-2657 0142-4319 |
| Popis: | Skeletal muscle atrophy as a consequence of acute and chronic illness, immobilisation, muscular dystrophies and aging, leads to severe muscle weakness, inactivity and increased mortality. Mechanical loading is thought to be the primary driver for skeletal muscle hypertrophy, however the extent to which mechanical loading can offset muscle catabolism has not been thoroughly explored. In vitro 3D-models of skeletal muscle provide a controllable, high throughput environment and mitigating many of the ethical and methodological constraints present during in vivo experimentation. This work aimed to determine if mechanical loading would offset dexamethasone (DEX) induced skeletal muscle atrophy, in muscle engineered using the C2C12 murine cell line. Mechanical loading successfully offset myotube atrophy and functional degeneration associated with DEX regardless of whether the loading occurred before or after 24 h of DEX treatment. Furthermore, mechanical load prevented increases in MuRF-1 and MAFbx mRNA expression, critical regulators of muscle atrophy. Overall, we demonstrate the application of tissue engineered muscle to study skeletal muscle health and disease, offering great potential for future use to better understand treatment modalities for skeletal muscle atrophy. Electronic supplementary material The online version of this article (10.1007/s10974-020-09589-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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