The FlbA-regulated predicted transcription factor Fum21 of Aspergillus niger is involved in fumonisin production
Autor: | Aerts, David, Hauer, Esther E., Ohm, Robin A., Arentshorst, Mark, Teertstra, Wieke R., Phippen, Christopher, Ram, Arthur F J, Frisvad, Jens C., Wösten, Han A. B., Sub Molecular Microbiology, Molecular Microbiology |
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Přispěvatelé: | Sub Molecular Microbiology, Molecular Microbiology |
Jazyk: | angličtina |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
endogenous compound 030106 microbiology Conidiation conidium Fumonisins Microbiology Fungal Proteins 03 medical and health sciences Gene Expression Regulation Fungal Gene expression protein secretion Asexual development deletion mutant Secondary metabolism Molecular Biology Transcription factor Gene transcription factor Original Paper Fungus Mycotoxin secondary metabolism nonhuman biology Strain (chemistry) Gene Expression Profiling Aspergillus niger fungi Fumonisin General Medicine biology.organism_classification 030104 developmental biology Aspergillus cytolysis gene expression cell wall mycelium RGS protein sporogenesis wild type Transcription Factor Gene Transcription Factors |
Zdroj: | Antonie van Leeuwenhoek, 111(3), 311-322 Aerts, D, Hauer, E E, Ohm, R A, Arentshorst, M, Teertstra, W R, Phippen, C, Ram, A F J, Frisvad, J C & Wösten, H A B 2018, ' The FlbA-regulated predicted transcription factor Fum21 of Aspergillus niger is involved in fumonisin production ', Antonie van Leeuwenhoek, vol. 111, no. 3, pp. 311-322 . https://doi.org/10.1007/s10482-017-0952-1 Antonie van Leeuwenhoek Antonie van Leeuwenhoek, 111, 311. Springer Netherlands Antonie Van Leeuwenhoek |
ISSN: | 0003-6072 |
Popis: | Aspergillus niger secretes proteins throughout the colony except for the zone that forms asexual spores called conidia. Inactivation of flbA that encodes a regulator of G-protein signaling results in colonies that are unable to reproduce asexually and that secrete proteins throughout the mycelium. In addition, the ΔflbA strain shows cell lysis and has thinner cell walls. Expression analysis showed that 38 predicted transcription factor genes are differentially expressed in strain ΔflbA. Here, the most down-regulated predicted transcription factor gene, called fum21, was inactivated. Growth, conidiation, and protein secretion were not affected in strain Δfum21. Whole genome expression analysis revealed that 63 and 11 genes were down- and up-regulated in Δfum21, respectively, when compared to the wild-type strain. Notably, 24 genes predicted to be involved in secondary metabolism were down-regulated in Δfum21, including 10 out of 12 genes of the fumonisin cluster. This was accompanied by absence of fumonisin production in the deletion strain and a 25% reduction in production of pyranonigrin A. Together, these results link FlbA-mediated sporulation-inhibited secretion with mycotoxin production. Electronic supplementary material The online version of this article (doi:10.1007/s10482-017-0952-1) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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