Applications of protein mass spectrometry in cell biology
| Autor: | Roberto Solari, Adele Rowley, Jyoti S. Choudhary, Walter P. Blackstock, Malcolm A. Ward, Malcolm Peter Weir, Martina Marzioch |
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| Rok vydání: | 2000 |
| Předmět: |
Enzymatic digestion
Protein mass spectrometry Sequence database Databases Factual Molecular Sequence Data Computational biology Biology Proteomics Mass spectrometry Molecular biology General Biochemistry Genetics and Molecular Biology DNA sequencing Protein expression Mass Spectrometry Peptide Fragments Sequence Analysis Protein Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Identification (biology) Amino Acid Sequence Molecular Biology Glycoproteins |
| Zdroj: | Methods (San Diego, Calif.). 20(4) |
| ISSN: | 1046-2023 |
| Popis: | Advances in mass spectrometry combined with accelerated progress in genome sequencing projects have facilitated the rapid identification of proteins by enzymatic digestion, mass analysis, and sequence database searching. Applications for this technology range from the surveillance of protein expression in cells, tissues, and whole organisms, to the identification of proteins and posttranslational modifications. Here we consider practical aspects of the application of mass spectrometry in cell biology and illustrate these with examples from our own laboratories. |
| Databáze: | OpenAIRE |
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