Xanthomonas citri pv. viticola Affecting Grapevine in Brazil: Emergence of a Successful Monomorphic Pathogen
| Autor: | Marco Aurélio Siqueira da Gama, Rosa de Lima Ramos Mariano, Sophie Cesbron, Sophie Bonneau, Marisa A. S. V. Ferreira, Maria Angélica Guimarães Barbosa, Martial Briand, Armelle Darrasse, Elineide Barbosa de Souza, Marie-Agnès Jacques, Perrine Portier |
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| Přispěvatelé: | Marisa A. S. V. Ferreira, Sophie Bonneau, Martial Briand, Sophie Cesbron, Perrine Portier, Armelle Darrasse, Marco A. S. Gama, MARIA ANGELICA GUIMARAES BARBOSA, CPATSA, Rosa de L. R. Mariano, Elineide B. Souza, Marie-Agnès Jacques., Universidade de Brasília (UnB), Institut de Recherche en Horticulture et Semences (IRHS), Université d'Angers (UA)-Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universidade Federal Rural de Pernambuco, Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Capes-MEC (Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior, Ministerio da Educacao), Brazil |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
0106 biological sciences 0301 basic medicine MLSA Plant Science 01 natural sciences Pathovar viticola Analyse de séquance multilocus (MLSA) Xanthomonas citri Disease control Genotype évolution Videira détection de clusters Disease and pest management 2. Zero hunger Genetics Vegetal Biology brésil biology Microbiology and Parasitology analyse phylogeographique Microbiologie et Parasitologie Agricultural sciences [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Uva Pathovar pullulation Patógeno Xanthomonas Citri Bio-informatique grapevine bacterial canker Grapes Bioinformatics 030106 microbiology MLVA Vitis vinifera Xanthomonas campestris pv. viticola Locus (genetics) Multiple Loci VNTR Analysis lcsh:Plant culture 03 medical and health sciences Tandem repeat [SDV.BV]Life Sciences [q-bio]/Vegetal Biology lcsh:SB1-1110 Typing Doença sélection génomique Cancro Bacteriano chancre bactérien structure génétique des populations Haplotype biology.organism_classification Bacterial canker Vitis Vinifera identification [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM] espèce apparentée vigne Biologie végétale Sciences agricoles 010606 plant biology & botany |
| Zdroj: | Frontiers in Plant Science (10), . (2019) Frontiers in Plant Science, Vol 10 (2019) Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA-Alice) Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA Frontiers in Plant Science Frontiers in Plant Science, Frontiers, 2019, 10, ⟨10.3389/fpls.2019.00489⟩ |
| ISSN: | 0618-0736 1664-462X |
| DOI: | 10.3389/fpls.2019.00489⟩ |
| Popis: | The pathovar viticola of Xanthomonas citri causes bacterial canker of grapevine. This disease was first recorded in India in 1972, and later in Brazil in 1998, where its distribution is currently restricted to the northeastern region. A multilocus sequence analysis (MLSA) based on seven housekeeping genes and a multilocus variable number of tandem repeat analysis (MLVA) with eight loci were performed in order to assess the genetic relatedness among strains from India and Brazil. Strains isolated in India from three related pathovars affecting Vitaceae species and pathogenic strains isolated from Amaranthus sp. found in bacterial canker-infected vineyards in Brazil were also included. MLSA revealed lack of diversity in all seven genes and grouped grapevine and Amaranthus strains in a monophyletic group in X. citri. The VNTR (variable number of tandem repeat) typing scheme conducted on 107 strains detected 101 haplotypes. The total number of alleles per locus ranged from 5 to 12. A minimum spanning tree (MST) showed that Brazilian strains were clearly separated from Indian strains, which showed unique alleles at three loci. The two strains isolated from symptomatic Amaranthus sp. presented unique alleles at two loci. STRUCTURE analyses revealed three groups congruent with MST and a fourth group with strains from India and Brazil. Admixture among populations were observed in all groups. MST, STRUCTURE and e-BURST analyses showed that the strains collected in 1998 belong to two distinct groups, with predicted founder genotypes from two different vineyards in the same region. This suggest that one introduction of grape planting materials contaminated with genetically distinct strains took place, which was followed by pathogen adaptation. Genome sequencing of one Brazilian strain confirmed typical attributes of pathogenic xanthomonads and allowed the design of a complementary VNTR typing cheme dedicated to X. citri pv. viticola that will allow further epidemiological survey of this genetically monomorphic pathovar. Made available in DSpace on 2019-11-29T18:07:54Z (GMT). No. of bitstreams: 1 Xanthomonascitripv.viticolaAffectingGrapevineinBrazil2019.pdf: 3096692 bytes, checksum: ec2b3ffb0c06180736f471cfff22e23f (MD5) Previous issue date: 2019 |
| Databáze: | OpenAIRE |
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