Improving the intein-mediated, site-specific protein biotinylation strategies both in vitro and in vivo
| Autor: | Shao Q. Yao, Rina Y. P. Lue, Lay Pheng Tan, Grace Y. J. Chen |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Microarray
Chemistry Green Fluorescent Proteins Organic Chemistry Clinical Biochemistry Protein Array Analysis Biotin Proteins Pharmaceutical Science Proteomics Biochemistry Protein biotinylation In vitro Inteins Cell biology In vivo Biotinylation Drug Discovery Protein microarray Molecular Medicine Cloning Molecular Intein Molecular Biology |
| Zdroj: | Bioorganic & Medicinal Chemistry Letters. 14:6067-6070 |
| ISSN: | 0960-894X |
| DOI: | 10.1016/j.bmcl.2004.09.083 |
| Popis: | One of the critical issues in the generation of a protein microarray lies in the choice of immobilization strategies, which ensure proteins are adhered to the glass surface while properly retaining their native biological activities. We previously developed intein-mediated strategies for protein biotinylation and site-specific protein microarray generation. Herein, we report new findings of these strategies, which improve the biotinylation efficiency of proteins by up to 10-folds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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