Metabolomic-Based Approach to Identify Biomarkers of Apple Intake
| Autor: | Diana González-Peña, Aoife E. McNamara, Cassandra Collins, Breige A. McNulty, Anne P. Nugent, Pedapati S. C. Sri Harsha, Albert Flynn, Janette Walton, Lorraine Brennan, Helena Gibbons |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Adult Male Multivariate analysis Magnetic Resonance Spectroscopy Physiology Urine Xylose Urinalysis 03 medical and health sciences chemistry.chemical_compound Metabolomics Clinical Research Food intake Medicine Humans Nutrition 2. Zero hunger Principal Component Analysis 030109 nutrition & dietetics Apples business.industry Prevention Middle Aged 030104 developmental biology chemistry Dietary assessment Malus Cohort Calibration Biomarker (medicine) Population study Female business Biomarkers Food Science Biotechnology Cohort study |
| Zdroj: | McNamara, A E, Collins, C, Harsha, P S C S, González-Peña, D, Gibbons, H, McNulty, B A, Nugent, A P, Walton, J, Flynn, A & Brennan, L 2020, ' Metabolomic-Based Approach to Identify Biomarkers of Apple Intake ', Molecular Nutrition and Food Research, vol. 64, no. 11, 1901158 . https://doi.org/10.1002/mnfr.201901158 |
| ISSN: | 1613-4133 |
| DOI: | 10.1002/mnfr.201901158 |
| Popis: | Scope: There is an increased interest in developing biomarkers of food intake to address some of the limitations associated with self-reported data. The objective is to identify biomarkers of apple intake, examine dose–response relationships, and agreement with self-reported data. Methods and results: Metabolomic data from three studies are examined: an acute intervention, a short-term intervention, and a free-living cohort study. Fasting and postprandial urine samples are collected for analysis by 1H-NMR and liquid chromatography–mass spectrometry (LC–MS). Calibration curves are developed to determine apple intake and classify individuals into categories of intake. Multivariate analysis of data reveals that levels of multiple metabolites increase significantly post-apple consumption, compared to the control food—broccoli. In the dose-response study, urinary xylose, epicatechin sulfate, and 2,6-dimethyl-2-(2-hydroxyethyl)-3,4-dihydro-2H-1-benzopyran increase as apple intake increases. Urinary xylose concentrations in a free-living cohort perform poorly at an individual level but are capable of ranking individuals in categories of intake. Conclusion: Urinary xylose exhibits a dose–response relationship with apple intake and performs well as a ranking biomarker in the population study. Other potential biomarkers are identified and future work will combine these with xylose in a biomarker panel which may allow for a more objective determination of individual intake. |
| Databáze: | OpenAIRE |
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