Immune Unresponsiveness by Intraportal UV-B-Irradiated Donor Antigen Administration Requires Persistence of Donor Antigen in a Nerve Allograft Model
| Autor: | Thomas H. Tung, M. Wayne Flye, Susan E. Mackinnon, Vaishali B. Doolabh, Daniel A. Hunter |
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| Rok vydání: | 2004 |
| Předmět: |
Graft Rejection
Ultraviolet Rays T-Lymphocytes Host tissue Persistence (computer science) Rats Sprague-Dawley Random Allocation Antigen Animals Transplantation Homologous Medicine Rats Inbred BB Peripheral Nerves Rats Inbred BUF Immune unresponsiveness Nerve allograft business.industry Regeneration (biology) Cytotoxicity Tests Immunologic Sciatic Nerve In vitro Nerve Regeneration Rats surgical procedures operative Rats Inbred Lew Immunology Immune reactivity Transplantation Tolerance Surgery business |
| Zdroj: | Journal of Reconstructive Microsurgery. 21:43-51 |
| ISSN: | 1098-8947 0743-684X |
| DOI: | 10.1055/s-2004-818049 |
| Popis: | The purpose of this study was to characterize the mechanism of unresponsiveness produced by the intraportal administration of ultraviolet-B (UV-B)-irradiated donor antigen. Pretreated Buffalo rats accepted Lewis nerve allografts, had decreased in vitro T-cell reactivity, and demonstrated nerve regeneration and recovery of limb function, while rejecting third-party nerve allografts. Regenerated nerve grafts were then retransplanted into a second naïve recipient. Rejection of the retransplanted allograft by naïve donor-strain, but not recipient-strain, animals suggests that the allografts were completely replaced by host tissue. Pretreated Buffalo rats were also given a second Lewis allograft after the first had regenerated. The second allograft was rejected and in vitro immune reactivity was comparable to naïve animals. Because the unresponsiveness state was extinguished with loss of exposure to donor antigen, these findings suggest that the intraportal administration of UV-B-irradiated donor antigen works by anergic or suppressive regulatory, rather than deletional, mechanisms. |
| Databáze: | OpenAIRE |
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