Effect of Blood Transfusion on Antigen Presentation Function and on Interleukin 2 Generation
| Autor: | Rabie N. Stephan, Richard E. Dean, Irshad H. Chaudry, Alexander S. Geha, John M. Kisala |
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| Rok vydání: | 1988 |
| Předmět: |
Male
Interleukin 2 medicine.medical_specialty Blood transfusion medicine.medical_treatment Antigen presentation Antigen-Presenting Cells Mice Inbred Strains Lymphocyte Activation Mice Immune system Immunity medicine Splenocyte Animals Blood Transfusion Lymphocytes biology business.industry Macrophages Surgery Cell culture Concanavalin A Immunology biology.protein Interleukin-2 business Spleen medicine.drug |
| Zdroj: | Archives of Surgery. 123:235 |
| ISSN: | 0004-0010 |
| Popis: | • To study the effect of blood transfusion (BT) on cell-mediated immunity, we examined the antigen presentation function of peritoneal macrophages and interleukin 2 (IL-2) generation by splenocytes. C3H/HEJ mice were transfused with 0.2 mL of fresh allogeneic blood obtained from C57BL/6 mice; they were killed on days 1, 3, and 7 after BT. A second group of C3H/HEJ mice was transfused with 0.2 mL/d of the same allogeneic blood on three successive days; they were killed on day 7 following the last BT. The antigen presentation function of peritoneal macrophages was measured by utilizing a D10.G4.1 T-helper cell clone; IL-2 activity in supernatants of concanavalin A—stimulated splenocytes was tested by utilizing an IL-2-dependent HT-2 cell line. The results indicate that although antigen presentation function remains unaffected after single and multiple BTs, the ability of splenocytes to generate IL-2 decreases significantly even after a single BT. Thus, the increased susceptibility to infection and the additional immune perturbations in malignant neoplasms following BT may be due in part to decreased IL-2 generation. ( Arch Surg 1988;123:235-240) |
| Databáze: | OpenAIRE |
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