Tissue-Specific RNA-Seq Analysis and Identification of Receptor-Like Proteins Related to Plant Growth in Capsicum annuum
| Autor: | Seon-In Yeom, Won-Hee Kang, Junesung Lee, Boseul Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine virus-induced gene silencing (VIGS) RNA-Seq Plant Science Plant disease resistance Biology 01 natural sciences Genome growth regulation functional analysis 03 medical and health sciences Capsicum spp Pepper Gene family Gene silencing Gene Ecology Evolution Behavior and Systematics Genetics Ecology fungi receptor-like protein (RLP) Botany food and beverages Phenotype 030104 developmental biology QK1-989 010606 plant biology & botany |
| Zdroj: | Plants, Vol 10, Iss 972, p 972 (2021) Plants Volume 10 Issue 5 |
| ISSN: | 2223-7747 |
| Popis: | Receptor-like proteins (RLPs) are a gene family of cell surface receptors that are involved in plant growth, development, and disease resistance. In a recent study, 438 pepper RLP genes were identified in the Capsicum annuum genome (CaRLPs) and determined to be present in response to multiple biotic stresses. To further understand the role of CaRLPs in plant growth and development, we analyzed expression patterns of all CaRLPs from various pepper tissues and developmental stages using RNA-seq. Ten CaRLP genes were selected for further analysis according to transcript levels with hierarchical clustering. The selected CaRLP genes displayed similarity of motifs within the same groups and structures typical of RLPs. To examine RLP function in growth and development, we performed loss-of-function analysis using a virus-induced gene silencing system. Three of the ten tested CaRLPs (CaRLP238, 253, and 360) in silenced plants exhibited phenotypic alteration with growth retardation compared to controls. All three gene-silenced peppers showed significant differences in root dry weight. Only CaRLP238 had significant differences in both root and shoot dry weight. Our results suggest that CaRLPs may play important roles in regulation of plant growth and development as well as function in defense responses to biotic stresses in the RLP gene family. |
| Databáze: | OpenAIRE |
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