An organ culture of adult mouse skin: An in vitro model for studying the molecular mechanism of skin tumor promotion
| Autor: | Ajit K. Verma, R.K. Boutwell |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Skin Neoplasms
Carboxy-Lyases Indomethacin Biophysics Retinoic acid Tretinoin Biology Ornithine Decarboxylase Organ culture Models Biological Biochemistry Ornithine decarboxylase HeLa Mice chemistry.chemical_compound Organ Culture Techniques Animals Molecular Biology Skin integumentary system DNA Neoplasms Experimental Cell Biology biology.organism_classification Phorbols Molecular biology chemistry Enzyme Induction Mouse skin Molecular mechanism Tetradecanoylphorbol Acetate Female Explant culture |
| Zdroj: | Biochemical and Biophysical Research Communications. 96:854-862 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(80)91434-5 |
| Popis: | A simple method to culture explants of adult mouse skin in a modified Eagle's HeLa cell medium was developed in order to further study the biochemical responses to the tumor promoting phorbol esters. The skin explants remained viable for at least 48 hr, as determined by their ability to incorporate 3H-thymidine into DNA as well as to induce epidermal ornithine decarboxylase (EC 4.1.1.17) activity following 12-0-tetradecanoylphorbol-13-acetate addition. The time course of induction of ornithine decarboxylase activity by the tumor promoter was similar to that observed with intact mice. Furthermore, the addition of retinoic acid and indomethacin, the agents that are known to inhibit the induction of ornithine decarboxylase activity by topically applied TPA, also inhibited the induction of ornithine decarboxylase activity by TPA in skin explants. |
| Databáze: | OpenAIRE |
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