Accumulation of mineral oil saturated hydrocarbons (MOSH) in female Fischer 344 rats: Comparison with human data and consequences for risk assessment

Autor: Laura Barp, Koni Grob, Unni Cecilie Nygaard, Florence Blas-Y-Estrada, Maurus Biedermann, Jean Pierre Cravedi, Jan Alexander
Přispěvatelé: Official Food Control Authority of the Canton of Zürich, ToxAlim (ToxAlim), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Ecole d'Ingénieurs de Purpan (INPT - EI Purpan), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA), Norwegian Institute of Public Health [Oslo] (NIPH), The research on rats was part of a grant project co-funded by the European Food Safety Authority (EFSA), grant agreement GP/EFSA/BIOCONTAM/2013/01 ('Bioaccumulation and toxicity of mineral oil hydrocarbons in rats - specificity of different subclasses of a broad mixture relevant for human dietary exposures'), Plateforme Ezop (Ezop), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Recherche Agronomique (INRA)-Université Toulouse III - Paul Sabatier (UT3)
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Biomagnification
[SDV]Life Sciences [q-bio]
Adipose tissue
tissu adipeux
comprehensive two-dimensional gas chromatography (GCxGC)
01 natural sciences
Rendering (animal products)
hydrocarbone
Tissue Distribution
rat
Food science
hydrocarbons
Mineral oil
Waste Management and Disposal
Chemistry
04 agricultural and veterinary sciences
foie
040401 food science
Pollution
adipose tissue
mineral oil
biomagnification
bioaccumulation
Liver
Bioaccumulation
Comprehensive two-dimensional gas chromatography
Female
medicine.drug
Environmental Engineering
molecular mass distribution
chromatographie bidimensionnelle
Risk Assessment
alkyl monocyclic
0404 agricultural biotechnology
chromatographie à phase gazeuse
medicine
Environmental Chemistry
Animals
Humans
Plant Oils
two dimensional chromatography
Chromatography
Molecular mass
010401 analytical chemistry
huile minérale
Rats
Inbred F344
class I mineral oil
rate
0104 chemical sciences
Rats
spleen
Gas chromatography
liver function tests
Zdroj: Science of the Total Environment
Science of the Total Environment, Elsevier, 2017, 575, pp.1263-1278. ⟨10.1016/j.scitotenv.2016.09.203⟩
ISSN: 0048-9697
1879-1026
DOI: 10.1016/j.scitotenv.2016.09.203⟩
Popis: Female Fischer 344 rats were orally exposed to a mixture of mineral oil saturated hydrocarbons (MOSH) of broad molecular mass range at doses of 40, 400 and 4000 mg/kg feed. Amounts and compositions of the MOSH were analyzed in liver, spleen, adipose tissue and the carcass after exposure during 30, 60, 90 and 120 d as well as after 90 d exposure followed by 30 d depuration. At 40 mg/kg in the feed, after 30 d of exposure, 10.9% of the ingested MOSH were recovered from the animal body; after 90 d plus 30 d depuration it was 3.9%. In liver and spleen, the maximum retention in terms of molecular mass (simulated distillation) was at n-C29; in adipose tissue and carcass it was at n-C15/16. The differentiation between MOSH below and above n-C25 (Class I versus Class II and III oils), used for present regulation, is not supported by the present data on accumulation; structural characteristics seem more pertinent than molecular mass. Concentrations in the tissues increased far less than proportionally with the dose, rendering linear extrapolation to low doses questionable. No steady state was reached after 120 d. In fact, comparing with the concentrations in human tissues at the estimated exposure, extrapolation from animal experiments seems to grossly underestimate human internal exposure. Comprehensive two-dimensional gas chromatography (GCxGC) was used to characterize the MOSH residues in the tissues with the aim of identifying the most strongly accumulated types. In the liver and spleen, the highly branched hydrocarbons dominated, whereas in the adipose tissue it was the n-alkanes and species with main n-alkyl moieties. Strong MOSH accumulation is not of concern per se, but the safety at the high concentrations in human tissues needs to be re-evaluated, possibly taking into account also end points other than granuloma formation.
Databáze: OpenAIRE
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