Alcohol Dehydrogenases and Acetaldehyde Dehydrogenases are Beneficial for Decidual Stromal Cells to Resist the Damage from Alcohol
| Autor: | Zi-Long Li, Yong-Sheng Fu, Shu-Yun Li, Meng-Qi Li, Zeng-Ming Yang |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Stromal cell Cell Survival Apoptosis Andrology 03 medical and health sciences chemistry.chemical_compound Internal medicine medicine Decidua Humans Viability assay Alcohol dehydrogenase Cell Line Transformed Ethanol biology Acetaldehyde Alcohol Dehydrogenase Decidualization General Medicine Aldehyde Oxidoreductases 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry biology.protein Female Stromal Cells |
| Zdroj: | Alcohol and alcoholism (Oxford, Oxfordshire). 52(2) |
| ISSN: | 1464-3502 |
| Popis: | Aims The aim of this study was to examine the effect of alcohol on the decidualization of human endometrial stromal cells during early pregnancy. Methods During in vitro decidualization, human endometrial stromal cells were treated with alcohol, 4-methylpyrazole hydrochloride (FPZ), the inhibitor of alcohol dehydrogenases (ADHs), and tetraethylthiuram disulfide (DSF), the inhibitor of acetaldehyde dehydrogenases (ALDHs), respectively. Cell viability and decidualization were examined. Apoptosis and proliferation were also evaluated. Results The findings showed that ADHs and ALDHs were up-regulated during decidualization. After alcohol treatment, the cell viability of decidual stromal cells was significantly higher than control, which was abrogated by FPZ or DSF. When cells were treated with alcohol, proliferation-related signal pathways were up-regulated in decidualized cells. Additionally, FOXO1 transcriptionally up-regulates ADH1B. Conclusion Our study provided an evidence that highly expressed ADHs and ALDHs endow decidual stromal cells an ability to alleviate the harm from alcohol. |
| Databáze: | OpenAIRE |
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