Freezing Weakens the Barrier Function of Reconstructed Human Epidermis as Evidenced by Raman Spectroscopy and Percutaneous Permeation
| Autor: | Christophe Eklouh-Molinier, Yuri Dancik, Franck Bonnier, Hichem Kichou, Emilie Munnier, Martin Soucé, Igor Chourpa |
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| Přispěvatelé: | Nanomédicaments et Nanosondes, EA 6295 (NMNS), Université de Tours (UT), LE STUDIUM (LE STUDIUM), Bureau de Recherches Géologiques et Minières (BRGM) (BRGM)-Centre national du machinisme agricole, du génie rural, des eaux et forêts (CEMAGREF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de recherche pour le développement [IRD] : UR-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tours |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Kinetics
Analytical chemistry Pharmaceutical Science lcsh:RS1-441 Resorcinol freezing Article lcsh:Pharmacy and materia medica storage 030207 dermatology & venereal diseases 03 medical and health sciences chemistry.chemical_compound symbols.namesake 0302 clinical medicine [CHIM.ANAL]Chemical Sciences/Analytical chemistry reconstructed human epidermis Stratum corneum medicine skin barrier resorcinol Barrier function 030304 developmental biology 0303 health sciences Aqueous solution Epidermis (botany) Chemistry Permeation medicine.anatomical_structure 13. Climate action Raman spectroscopy symbols permeation EpiSkin™ |
| Zdroj: | Pharmaceutics Volume 12 Issue 11 Pharmaceutics, MDPI, 2020, 12 (11), pp.1041. ⟨10.3390/pharmaceutics12111041⟩ Pharmaceutics, Vol 12, Iss 1041, p 1041 (2020) |
| ISSN: | 1999-4923 |
| DOI: | 10.3390/pharmaceutics12111041 |
| Popis: | The development and characterization of reconstructed human epidermis (RHE) is an active area of R& D. RHE can replace animal tissues in pharmaceutical, toxicological and cosmetic sciences, yielding scientific and ethical advantages. RHEs remain costly, however, due to consumables and time required for their culture and a short shelf-life. Storing, i.e., freezing RHE could help reduce costs but to date, little is known on the effects of freezing on the barrier function of RHE. We studied such effects using commercial EpiSkin&trade RHE stored at &minus 20, &minus 80 and &minus 150 ° C for 1 and 10 weeks. We acquired intrinsic Raman spectra in the stratum corneum (SC) of the RHEs as well as spectra obtained following topical application of resorcinol in an aqueous solution. In parallel, we quantified the effects of freezing on the permeation kinetics of resorcinol from time-dependent permeation experiments. Principal component analyses discriminated the intrinsic SC spectra and the spectra of resorcinol-containing RHEs, in each case on the basis of the freezing conditions. Permeation of resorcinol through the frozen RHE increased 3- to 6-fold compared to fresh RHE, with the strongest effect obtained from freezing at &minus 20 ° C for 10 weeks. Due to the extensive optimization and standardization of EpiSkin&trade RHE, the effects observed in our work may be expected to be more pronounced with other RHEs. |
| Databáze: | OpenAIRE |
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