DNA methylation inhibits expression and transposition of the Neurospora Tad retrotransposon
| Autor: | John A. Kinsey, Y Zhou, E B Cambareri |
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| Rok vydání: | 2001 |
| Předmět: |
Retroelements
Recombinant Fusion Proteins Retrotransposon Biology Neurospora crassa Transposition (music) chemistry.chemical_compound Genes Reporter Gene Expression Regulation Fungal Genetics Escherichia coli Gene Silencing DNA Fungal Molecular Biology Regulation of gene expression fungi Fungal genetics General Medicine Methylation DNA Methylation biology.organism_classification chemistry Lac Operon DNA methylation Azacitidine DNA |
| Zdroj: | Molecular genetics and genomics : MGG. 265(4) |
| ISSN: | 1617-4615 |
| Popis: | Tad is a LINE-like retrotransposon of the filamentous fungus Neurospora crassa. We have analyzed both expression and transposition of this element using strains with a single copy of Tad located in the 5' noncoding sequences of the am (glutamate dehydrogenase) gene. Tad in this position has been shown to carry a de novo cytosine methylation signal which causes reversible methylation of both Tad and am upstream sequences. Here we find that methylation of the Tad sequences inhibits both Tad expression and transposition. This inhibition can be relieved by the use of 5-azacytidine, a drug which reduces cytosine methylation, or by placing the Tad/am sequences in a dim-2 genetic background. |
| Databáze: | OpenAIRE |
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