Data-independent proteome analysis of ARPE-19 cells
| Autor: | Francesco Giorgianni, Diwa Koirala, Sarka Beranova-Giorgianni |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Proteomics Multidisciplinary Chromatography 030102 biochemistry & molecular biology Chemistry Quantitative proteomics Fractionation lcsh:Computer applications to medicine. Medical informatics Mass spectrometry Retinal pigment epithelial cell High-performance liquid chromatography In vitro 03 medical and health sciences 030104 developmental biology Proteome lcsh:R858-859.7 lcsh:Science (General) lcsh:Q1-390 |
| Zdroj: | Data in Brief Data in Brief, Vol 20, Iss, Pp 333-336 (2018) |
| ISSN: | 2352-3409 |
| Popis: | We have performed a proteomics analysis of a human retinal pigment epithelial cell line (ARPE-19), which represents a widely used model for in vitro studies of cellular and molecular mechanisms related to human RPE cells (Dunn et al., 1996; Weigel et al., 2002) [1] , [2] . Whole cell protein extracts were separated in four gel fractions via short (10 min) SDS-PAGE runs. Following fractionation and trypsin digestion, the resulting peptides were separated on a nano UPLC LC system and analyzed on-line with a QTof-IMS instrument: a tandem mass spectrometer with ion mobility separation (Synapt G2-Si). Data were acquired in data-independent mode (UDMSE), which allows for absolute and/or relative post-acquisition protein quantification (Silva et al., 2006) [3] . The proteome profile data obtained from this study can be used as a protein reference database with qualitative and quantitative protein information related to ARPE-19 cells under normal growth conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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