Evaluation of a Rapid Antigen Test To Detect SARS-CoV-2 Infection and Identify Potentially Infectious Individuals
| Autor: | Lutz Gieselmann, Nico Pfeifer, Eva Heger, Henning Gruell, Maike Wirtz, Gerd Fätkenheuer, Irina Fish, Kanika Vanshylla, M. Korenkov, Nareshkumar Poopalasingam, Florian Klein, Felix Dewald, Clara Lehmann, Ralf Eggeling, Matthias Madler |
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| Rok vydání: | 2021 |
| Předmět: |
Microbiology (medical)
Coronavirus disease 2019 (COVID-19) business.industry Viral culture SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RT-qPCR virus culture COVID-19 Real-Time Polymerase Chain Reaction Virology Communicable Diseases Sensitivity and Specificity Virus antigen test Real-time polymerase chain reaction Antigen Rapid antigen test Vero cell Medicine Humans infectiousness business |
| Zdroj: | Journal of Clinical Microbiology |
| ISSN: | 1098-660X |
| Popis: | The identification and isolation of highly infectious SARS-CoV-2-infected individuals is an important public health strategy. Rapid antigen detection tests (RADT) are promising tools for large-scale screenings due to timely results and feasibility for on-site testing. Nonetheless, the diagnostic performance of RADT in detecting infectious individuals is not yet fully determined. In this study, RT-qPCR and virus culture of RT-qPCR-positive samples were used to evaluate and compare the performance of the Standard Q COVID-19 Ag test in detecting SARS-CoV-2-infected and possibly infectious individuals. To this end, two combined oro- and nasopharyngeal swabs were collected at a routine SARS-CoV-2 diagnostic center. A total of 2,028 samples were tested, and 118 virus cultures were inoculated. SARS-CoV-2 infection was detected in 210 samples by RT-qPCR, representing a positive rate of 10.36%. The Standard Q COVID-19 Ag test yielded a positive result in 92 (4.54%) samples resulting in an overall sensitivity and specificity of 42.86 and 99.89%, respectively. For adjusted CT values of |
| Databáze: | OpenAIRE |
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