Suppression of RNA-dependent RNA polymerase 6 in tomatoes allows potato spindle tuber viroid to invade basal part but not apical part including pluripotent stem cells of shoot apical meristem

Autor: Syoya Kitabayashi, Takashi Naoi, Atsushi Kasai, Charith Raj Adkar-Purushothama, Tatsuji Hataya, Kohei Sugawara, Mineo Senda, Teruo Sano
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
Time Factors
Molecular biology
Physiology
Viroid
Nicotiana benthamiana
Plant Science
Genetically Modified Plants
Biochemistry
01 natural sciences
Meristems
RNA interference
Solanum lycopersicum
Gene Expression Regulation
Plant
Plant Proteins
Multidisciplinary
biology
Genetically Modified Organisms
Plant Anatomy
Eukaryota
food and beverages
Plants
Small interfering RNA
Plants
Genetically Modified
Viroids
Cell biology
Nucleic acids
RNA silencing
Genetic interference
Plant Physiology
Viruses
Seeds
Engineering and Technology
Medicine
RNA hybridization
Epigenetics
Genetic Engineering
Research Article
Biotechnology
Pluripotent Stem Cells
Science
Meristem
Plant Pathogens
RNA-dependent RNA polymerase
Bioengineering
Genome
Viral
Fruits
03 medical and health sciences
Tomatoes
Genetics
RNA
Messenger
Non-coding RNA
Potato spindle tuber viroid
Molecular probe techniques
fungi
Organisms
Biology and Life Sciences
RNA
Plant Pathology
Stem Anatomy
RNA-Dependent RNA Polymerase
biology.organism_classification
Probe hybridization
Gene regulation
Research and analysis methods
Molecular biology techniques
Shoot Apical Meristem
030104 developmental biology
Plant Biotechnology
Gene expression
010606 plant biology & botany
Zdroj: PLoS ONE, Vol 15, Iss 7, p e0236481 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: RNA-dependent RNA polymerase 6 (RDR6) is one of the key factors in plant defense responses and suppresses virus or viroid invasion into shoot apical meristem (SAM) in Nicotiana benthamiana. To evaluate the role of Solanum lycopersicum (Sl) RDR6 upon viroid infection, SlRDR6-suppressed (SlRDR6i) ‘Moneymaker’ tomatoes were generated by RNA interference and inoculated with intermediate or lethal strain of potato spindle tuber viroid (PSTVd). Suppression of SlRDR6 did not change disease symptoms of both PSTVd strains in ‘Moneymaker’ tomatoes. Analysis of PSTVd distribution in shoot apices by in situ hybridization revealed that both PSTVd strains similarly invade the basal part but not apical part including pluripotent stem cells of SAM in SlRDR6i plants at a low rate unlike a previous report in N. benthamiana. In addition, unexpectedly, amount of PSTVd accumulation was apparently lower in SlRDR6i plants than in control tomatoes transformed with empty cassette in early infection especially in the lethal strain. Meanwhile, SlRDR6 suppression did not affect the seed transmission rates of PSTVd. These results indicate that RDR6 generally suppresses PSTVd invasion into SAM in plants, while suppression of RDR6 does not necessarily elevate amount of PSTVd accumulation. Additionally, our results suggest that host factors such as RDR1 other than RDR6 may also be involved in the protection of SAM including pluripotent stem cells from PSTVd invasion and effective RNA silencing causing the decrease of PSTVd accumulation during early infection in tomato plants.
Databáze: OpenAIRE
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