Oral administration of supplementary biotin differentially influences the fertility rate and oviductal expression of avidin and avidin-related protein-2 in low- and high-fertility broiler line hens
| Autor: | H. Daryabari, Mohammad Javad Zamiri, Hamid Deldar, Amir Akhlaghi, G. Rahimi Mianji, Z. Ansari Pirsaraei, A.N. Eghbalian |
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| Rok vydání: | 2015 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment media_common.quotation_subject Administration Oral Biotin Fertility Oviducts Andrology chemistry.chemical_compound Oral administration Internal medicine medicine Animals RNA Messenger media_common biology Artificial insemination Broiler General Medicine Avidin Sperm Egg Yolk Endocrinology chemistry Gene Expression Regulation Dietary Supplements Vitamin B Complex biology.protein Animal Science and Zoology Female Reproduction Chickens |
| Zdroj: | Poultry science. 94(2) |
| ISSN: | 0032-5791 |
| Popis: | Probable involvement of avidin and avidin-related protein-2 (AVR2) in sperm viability in the sperm storage tubules of turkeys has been suggested. The high affinity of biotin to avidin and its analogs is also well documented. The present study aimed to determine the effect of oral biotin on reproductive performance and oviductal mRNA expression of avidin and AVR2 in 2 broiler hen lines with different fertility rates. Low-fertility (line B) and high-fertility (line D) hens (n=144) were randomly allotted to receive 0 (T0), 0.30 (T1), or 0.45 (T2) mg/L biotin in drinking water from 30 through 33 wk of age. The reproductive performance of the hens was evaluated using artificial insemination. At the end of the treatment period, 24 hens per line were killed to assay the expression of avidin and AVR2 in the uterovaginal junction. Supplementary biotin increased egg production from 73.5% for T0 to 87.8% for T2. Hens administered with biotin in line B, but not in line D, showed an increase (8.4%) in fertility rate. Hatchability, chick quality, and overall embryonic mortality were not different among the experimental groups. Real-time PCR data showed that both avidin (P=0.0013) and AVR2 (P |
| Databáze: | OpenAIRE |
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