The efficacy of Cot-based gene enrichment in wheat (Triticum aestivum L.)
| Autor: | Daniel G. Peterson, John P. Fellers, Bikram S. Gill, Didier Lamoureux, Wanlong Li |
|---|---|
| Přispěvatelé: | Génétique Diversité et Ecophysiologie des Céréales (GDEC), Institut National de la Recherche Agronomique (INRA)-Université Blaise Pascal - Clermont-Ferrand 2 (UBP), Department of Plant and Soil Sciences, Mississippi State University [Mississippi], Department of Plant Pathology, Wheat Genetics Resource Center, Kansas State University, ARS, Agricultural Research Service, United States Department of Agriculture, Department of Plant Pathology, USDA-ARS : Agricultural Research Service, Université Blaise Pascal - Clermont-Ferrand 2 (UBP)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
0106 biological sciences
RENATURATION KINETICS Genes Plant 01 natural sciences Genome 03 medical and health sciences Cot filtration chemistry.chemical_compound Predictive Value of Tests Genetics Aegilops tauschii Genomic library BANQUE DE GENES Cloning Molecular Repeated sequence Molecular Biology Gene Triticum Repetitive Sequences Nucleic Acid 030304 developmental biology 2. Zero hunger 0303 health sciences [SDV.GEN]Life Sciences [q-bio]/Genetics biology Shotgun sequencing food and beverages BREAD WHEAT Sequence Analysis DNA General Medicine biology.organism_classification chemistry GENE ENRICHMENT Genome Plant DNA 010606 plant biology & botany Biotechnology GENE RICH REGIONS |
| Zdroj: | Genome Genome, NRC Research Press, 2005, 48 (6), pp.1120-1126. ⟨10.1139/g05-080⟩ |
| ISSN: | 0831-2796 1480-3321 |
| DOI: | 10.1139/g05-080⟩ |
| Popis: | We report the results of a study on the effectiveness of Cot filtration (CF) in the characterization of the gene space of bread wheat (Triticum aestivum L.), a large genome species (1C = 16 700 Mb) of tremendous agronomic importance. Using published Cot data as a guide, 2 genomic libraries for hexaploid wheat were constructed from the single-stranded DNA collected at Cot values > 1188 and 1639 M·s. Compared with sequences from a whole genome shotgun library from Aegilops tauschii (the D genome donor of bread wheat), the CF libraries exhibited 13.7-fold enrichment in genes, 5.8-fold enrichment in unknown low-copy sequences, and a 3-fold reduction in repetitive DNA. CF is twice as efficient as methylation filtration at enriching wheat genes. This research suggests that, with improvements, CF will be a highly useful tool in sequencing the gene space of wheat.Key words: gene enrichment, renaturation kinetics, gene-rich regions, bread wheat. |
| Databáze: | OpenAIRE |
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