Apoptosis in idiopathic inflammatory myopathies with partial invasion; a role for CD8(+)cytotoxic T cells?
| Autor: | Jan Ernerudh, Liv Gröntoft, Bo Häggqvist, Olof Danielsson, Karin Öllinger |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
Pathology Biopsy Cell- och molekylärbiologi Muscle Fibers Skeletal Cell Membranes Apoptosis Polymyositis Cohort Studies White Blood Cells 0302 clinical medicine Animal Cells Cellular types Medicine and Health Sciences Cytotoxic T cell Immune Response Staining Multidisciplinary TUNEL assay Cell Death Chemistry Cell Staining Middle Aged Fas receptor Membrane Staining Cell Processes Medicine Female Cellular Structures and Organelles Research Article Adult medicine.medical_specialty Cell biology Blood cells Science Immune Cells Immunology T cells Cytotoxic T cells Surgical and Invasive Medical Procedures Research and Analysis Methods Myositis Inclusion Body 03 medical and health sciences Signs and Symptoms medicine Humans Aged 030203 arthritis & rheumatology Inflammation Biology and life sciences Macrophages Dermatomyositis medicine.disease Granzyme B Specimen Preparation and Treatment Inclusion body myositis Clinical Medicine 030217 neurology & neurosurgery Cell and Molecular Biology T-Lymphocytes Cytotoxic |
| Zdroj: | PLoS ONE PLoS ONE, Vol 15, Iss 9, p e0239176 (2020) |
| Popis: | Polymyositis and inclusion body myositis are idiopathic inflammatory myopathies, with a pathology characterized by partial invasion of non-necrotic muscle fibres by CD8(+)cytotoxic T-cells, leading to fibre degeneration. Although the main effector pathway of CD8(+)T-cells is to induce apoptosis of target cells, it has remained unclear if apoptosis occurs in these diseases, and if so, if it is mediated by CD8(+)T-cells. In consecutive biopsy sections from 10 patients with partial invasion, muscle fibres and inflammatory cells were assessed by immunohistochemistry and apoptotic nuclei by the TUNEL assay. Analysis of muscle fibre morphology, staining pattern and quantification were performed on digital images, and they were compared with biopsies from 10 dermatomyositis patients and 10 controls without muscle disease. Apoptotic myonuclei were found in muscle with partial invasion, but not in the invaded fibres. Fibres with TUNEL positive nuclei were surrounded by CD8(+)T-cells, granzyme B(+)cells and macrophages, but lacked FAS receptor expression. In contrast, apoptotic myonuclei were rare in dermatomyositis and absent in controls. The findings confirm that apoptosis occurs in idiopathic inflammatory myopathies and support that it is mediated by CD8(+)cytotoxic T- cells, acting in parallel to the process of partial invasion. Funding Agencies|Region Ostergotland [LIO-535621] |
| Databáze: | OpenAIRE |
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