Co-circulation of different A. phagocytophilum variants within cattle herds and possible reservoir role for cattle
| Autor: | Clotilde Rouxel, Henri-Jean Boulouis, Maëllys Kevin, Thibaud Dugat, Martin Pfeffer, Guillaume Girault, Anne-Claire Lagrée, Nadia Haddad, Benoit Durand, Marion Nieder, Cornelia Silaghi |
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| Přispěvatelé: | Biologie moléculaire et immunologie parasitaires et fongiques (BIPAR), Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Institut National de la Recherche Agronomique (INRA)-École nationale vétérinaire d'Alfort (ENVA)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université Paris-Est (COMUE), Partenaires INRAE, Institut National de la Recherche Agronomique (INRA), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Unité Epidémiologie, Leipzig University, Institute of Infectology, Federal Research Institute for Animal Health - Friedrich-Loeffler-Institut, French Ministry of Agriculture, French Agency for Food, Environmental and Occupational Health and Safety (Anses), French National Institute for Agricultural Research (INRA), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Laboratoire de santé animale, sites de Maisons-Alfort et de Dozulé, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Haddad, Nadia |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Veterinary medicine Disease reservoir animal diseases Cattle Diseases Germany Genotype 2. Zero hunger Tick-borne disease biology Coinfection Microbiology and Parasitology Bovine Microbiologie et Parasitologie Infectious Diseases Blood [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology Anaplasma phagocytophilum France MLST Reservoir Veterinary medicine and animal Health DNA Bacterial 030106 microbiology Real-Time Polymerase Chain Reaction lcsh:Infectious and parasitic diseases 03 medical and health sciences medicine Animals lcsh:RC109-216 Disease Reservoirs [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health Research Ehrlichiosis Genetic Variation medicine.disease biology.organism_classification 030104 developmental biology Médecine vétérinaire et santé animal Genetic marker Herd Multilocus sequence typing Parasitology Cattle Multilocus Sequence Typing |
| Zdroj: | Parasites and Vectors Parasites and Vectors, BioMed Central, 2018, 11, pp.1-10. ⟨10.1186/s13071-018-2661-7⟩ Parasites & Vectors Parasites and Vectors (11), 1-10. (2018) Parasites & Vectors, Vol 11, Iss 1, Pp 1-10 (2018) |
| ISSN: | 1756-3305 |
| Popis: | International audience; Background: Anaplasma phagocytophilum is a zoonotic tick-borne intracellular alpha-proteobacterium causing tick-borne fever, which leads to significant economic losses in domestic ruminants in Europe. Its epidemiological cycles are complex and reservoir host species of bovine strains have not yet been identified. Given that little genetic information is available on strains circulating within a defined bovine environment, our objective was to assess the genetic diversity of A. phagocytophilum obtained from the same farms over time. Methods: Blood samplings were performed several times in two European herds. In the French herd, 169 EDTA-blood samples were obtained from 115 cows (32 were sampled two to four times). In the German herd, 20 cows were sampled six times (120 EDTA-blood samples). The presence of A. phagocytophilum DNA was assessed using a qPCR targeting msp2. The positive DNA samples underwent MLST at nine genetic markers (typA, ctrA, msp4, pleD, recG, polA, groEL, gyrA, and ankA). For each locus, sequences were aligned with available bacterial sequences derived from cattle, horse, dog, and roe deer hosts, and concatenated neighbor joining trees were constructed using three to six loci. Results: Around 20% (57/289) of samples were positive. Forty positive samples from 23 French and six German cows (11 of them being positive at two time points) were sequenced. Six loci (typA, ctrA, msp4, pleD, recG, and polA) allowed to build concatenated phylogenetic trees, which led to two distinct groups of bovine variants in the French herd (hereafter called A and B), whereas only group A was detected in the German herd. In 42% of French samples, double chromatogram peaks were encountered in up to four loci. Eleven cows were found infected three weeks to 17 months after first sampling and harboured a new variant belonging to one or the other group. Conclusions: Our results demonstrate the occurrence of two major bovine strain groups and the simultaneous infection of single cows by more than one A. phagocytophilum strain. This challenges the role of cattle as reservoirs for A. phagocytophilum. This role may be facilitated via long-term bacterial persistence in individual cows and active circulation at the herd scale. |
| Databáze: | OpenAIRE |
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